PXD045337

PXD045337 is an original dataset announced via ProteomeXchange.

Title	Unveiling the Development Specific Regenerative Potential of Spinal Cord: Insights from Extracellular Matrix
Description	Neonatal spinal cord tissues exhibit remarkable regenerative capabilities compared to adult tissues following injury. Although some cellular signaling pathways involved in the process have been identified, the specific role of extracellular matrix (ECM) responsible for neonatal spinal cord regeneration has remained elusive. Here we revealed that early developmental spinal cord contained a higher abundance of ECM proteins associated with neural development and axon growth but fewer inhibitory proteoglycans compared to adult spinal cord. Decellularized spinal cord ECM from neonatal (DNSCM) and adult (DASCM) rabbits preserve the major difference of native spinal cord tissues in both stages. Compared to DASCM, DNSCM promoted proliferation, migration, and neuronal differentiation of neural progenitor cells (NPCs), as well as facilitated the long-distance axonal outgrowth and axon regeneration of spinal cord organoids. Pleiotrophin (PTN) and Tenascin (TNC) in DNSCM were identified as contributors to the remarkable neural regeneration ability. Furthermore, DNSCM demonstrated superior performance when used as a delivery vehicle for NPCs and organoids in rats with spinal cord injury (SCI). It suggests that ECM cues derived from different development stage might contribute to the distinct regeneration ability of spinal cord.
HostingRepository	iProX
AnnounceDate	2023-09-13
AnnouncementXML	Submission_2024-02-27_23:14:24.140.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zheng Sun
SpeciesList	scientific name: Oryctolagus cuniculus; NCBI TaxID: 9986;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-12 21:51:27	ID requested
⏵ 1	2024-02-27 23:14:24	announced

Dataset with its publication pending

submitter keyword: spinal cord injury, Extracellular matrix, axon regeneration, organoid

Yannan Zhao
contact affiliation	Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
contact email	ynzhao@genetics.ac.cn
lab head
Zheng Sun
contact affiliation	Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
contact email	sunzheng@genetics.ac.cn
dataset submitter

iProX dataset URI