PXD045334

PXD045334 is an original dataset announced via ProteomeXchange.

Title	Identification of interacting proteins with the NP protein of the non-oncolytic H-NP366-489I strain and the non-oncolytic Herts/33 strain by mass spectrometry
Description	Newcastle disease virus (NDV) is one of the most significant threats to the poultry industry, and it also exhibits oncolytic properties. In our research, we have identified a non-oncolytic strain, the genotype VII NDV strain I4. We have found that the lack of oncolytic characteristics in this strain is attributed to amino acids 366-489 in the NP protein. To delve into the mechanism of NP-mediated NDV oncolysis, we initiated further investigations using the highly oncolytic Herts/33 strain's NP and a recombinant NP protein with amino acids 366-489 from the I4 strain substituted in (H-NP366-489I). These proteins represent the interacting partners in the proteomics analysis of NP proteins from both viral strains. After infecting HeLa cells with each of these strains, immunoprecipitation was performed using antibodies targeting the NP protein. Since the H-NP366-489I virus produces fewer proteins during replication, we additionally overexpressed the H-NP366-489I and Herts/33 NP proteins separately for immunoprecipitation experiments.
HostingRepository	iProX
AnnounceDate	2023-09-12
AnnouncementXML	Submission_2023-09-12_19:14:18.286.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tianxing Liao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-12 19:13:43	ID requested
⏵ 1	2023-09-12 19:14:18	announced

Dataset with its publication pending

submitter keyword: mass spectrometry, Newcastle disease virus, NP protein, oncolytic virus

Xiufan Liu
contact affiliation	Yangzhou University
contact email	xfliu@yzu.edu.cn
lab head
Tianxing Liao
contact affiliation	Yangzhou University
contact email	2693040819@qq.com
dataset submitter

iProX dataset URI