PXD045316

PXD045316 is an original dataset announced via ProteomeXchange.

Title	O-GlcNAcylation in mitochondrial protein in rat astrocytes
Description	To further investigate this idea that O-GlcNAcylation in mitochondria can improve the efficacy and benefit of mitochondrial transfer, we asked what mitochondrial proteins were modified with O-GlcNAc. To identify O-GlcNAcylated proteins and the potential target sites of O-GlcNAcylation, rat astrocytes were stimulated with NAD to induce O-GlcNAcylation in mitochondria, then we isolated mitochondria and extracted O-GlcNAcylated proteins by immunoprecipitation using O-GlcNAc antibody followed by silver staining.
HostingRepository	PRIDE
AnnounceDate	2023-10-20
AnnouncementXML	Submission_2023-10-20_11:11:41.286.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045316
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	KazuhideHayakawa
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-12 09:30:58	ID requested
⏵ 1	2023-10-20 11:11:41	announced

10.6019/PXD045316;

submitter keyword: astrocytes,O-GlcNAcylation, mitochondria

KazuhideHayakawa
contact affiliation	Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts, USA
contact email	khayakawa1@mgh.harvard.edu
lab head
KazuhideHayakawa
contact affiliation	Department of Radiology, neuroprotection research laboratory
contact email	khayakawa1@mgh.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045316
     1. Label: PRIDE project
     2. Name: O-GlcNAcylation in mitochondrial protein in rat astrocytes