PXD045316

PXD045316 is an original dataset announced via ProteomeXchange.

Title	O-GlcNAcylation in mitochondrial protein in rat astrocytes
Description	To further investigate this idea that O-GlcNAcylation in mitochondria can improve the efficacy and benefit of mitochondrial transfer, we asked what mitochondrial proteins were modified with O-GlcNAc. To identify O-GlcNAcylated proteins and the potential target sites of O-GlcNAcylation, rat astrocytes were stimulated with NAD to induce O-GlcNAcylation in mitochondria, then we isolated mitochondria and extracted O-GlcNAcylated proteins by immunoprecipitation using O-GlcNAc antibody followed by silver staining.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:06:40.666.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045316
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kazuhide Hayakawa
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-12 09:30:58	ID requested
1	2023-10-20 11:11:41	announced
⏵ 2	2024-10-22 06:06:41	announced	2024-10-22: Updated project metadata.

10.6019/PXD045316;

submitter keyword: astrocytes,O-GlcNAcylation, mitochondria

Kazuhide Hayakawa
contact affiliation	Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts, USA
contact email	khayakawa1@mgh.harvard.edu
lab head
Kazuhide Hayakawa
contact affiliation	Department of Radiology, neuroprotection research laboratory
contact email	khayakawa1@mgh.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD045316

PRIDE project URI

• PRIDE
  1. PXD045316
     1. Label: PRIDE project
     2. Name: O-GlcNAcylation in mitochondrial protein in rat astrocytes