PXD045310

PXD045310 is an original dataset announced via ProteomeXchange.

Title	Yeast PKA holoenzyme and condensate targeted and label-free proteomics
Description	Protein kinase A (PKA) is a conserved kinase essential for many biological processes linked to growth, development and metabolism. The PKA catalytic subunit is expressed as multiple isoforms in diverse eukaryotes; however, their contribution to ensuring specificity in response to environmental cues remains poorly defined. In this study, we aimed to dissect the role of the Saccharomyces cerevisiae PKA isoforms, Tpk1-3, in the thermal stress response. We used an absolute quantification mass spectrometry approach to investigate stress-induced changes in the stoichiometry of Tpk1-3 bound to the PKA regulatory subunit. We identified Tpk3 accumulation in cytoplasmic granules upon stress and used immunopurification combined with proteomics to characterise the constituents of these Tpk3 granules.
HostingRepository	PRIDE
AnnounceDate	2025-09-19
AnnouncementXML	Submission_2025-09-19_09:01:41.590.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Declan Creamer
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-12 07:43:37	ID requested
⏵ 1	2025-09-19 09:01:41	announced

10.1016/J.CELREP.2024.114360;

submitter keyword: holoenzyme,Yeast, condensates, heat stress, PKA isoforms

Chris Grant
contact affiliation	Faculty of Biology, Medicine and Health, The University of Manchester
contact email	chris.grant@manchester.ac.uk
lab head
Declan Creamer
contact affiliation	Faculty of Biology, Medicine and Health, The University of Manchester
contact email	declan.creamer@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD045310

PRIDE project URI

• PRIDE
  1. PXD045310
     1. Label: PRIDE project
     2. Name: Yeast PKA holoenzyme and condensate targeted and label-free proteomics