PXD045285

PXD045285 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Periostin is a disulfide bonded homodimer and forms a complex with fibronectin in human skin
Description	The protein periostin is a matricellular protein that is expressed in connective tissue. It is composed of five globular domains arranged in an elongated structure with an extensive disordered C-terminal tail. Periostin contains eleven cysteine residues, of which one is unpaired, and the rest forms five intra-molecular disulfide bonds. Periostin plays an important role during wound healing and is also involved in driving the inflammatory state in atopic diseases. This study provides a comprehensive biochemical characterization of periostin in human skin and in dermal and pulmonary fibroblasts in vitro. Through the application of Western blotting, co-immunoprecipitation, and LC-MS/MS, we show for the first time that periostin is a disulfide bonded homodimer and engages in a novel disulfide bonded complex with fibronectin both in vivo and in vitro. This inherent characteristic of periostin holds the potential to redefine our approach to exploring and understanding its functional role in future research endeavors.
HostingRepository	PRIDE
AnnounceDate	2025-01-22
AnnouncementXML	Submission_2025-01-22_00:14:19.938.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Rusbjerg-Weberskov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-11 23:18:31	ID requested
⏵ 1	2025-01-22 00:14:20	announced

Publication List

Rusbjerg-Weberskov CE, Scavenius C, Enghild JJ, Nielsen NS, Periostin Is a Disulfide-Bonded Homodimer and Forms a Complex with Fibronectin in the Human Skin. Biochemistry, 63(20):2658-2669(2024) [pubmed]
10.1021/acs.biochem.4c00240;

Rusbjerg-Weberskov CE, Scavenius C, Enghild JJ, Nielsen NS, Periostin Is a Disulfide-Bonded Homodimer and Forms a Complex with Fibronectin in the Human Skin. Biochemistry, 63(20):2658-2669(2024) [pubmed]

10.1021/acs.biochem.4c00240;

Keyword List

submitter keyword: wound healing,Periostin, fibronectin, extracellular matrix

submitter keyword: wound healing,Periostin, fibronectin, extracellular matrix

Contact List

Jan J.
contact affiliation	Department of Molecular Biology and Genetics, Aarhus University, Denmark
contact email	jje@mbg.au.dk
lab head
Christian Rusbjerg-Weberskov
contact affiliation	Aarhus University
contact email	christianew@mbg.au.dk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD045285
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD045285

PRIDE project URI

Repository Record List

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