PXD045279

PXD045279 is an original dataset announced via ProteomeXchange.

Title	Hepatic Huwe1 loss protects mice from non-alcoholic fatty liver disease through lipid metabolic rewiring
Description	Non-alcoholic fatty liver disease (NAFLD) is the most prevalent form of liver disease worldwide and is estimated to affect nearly a third of the population. Huwe1, also known as ARF-BP1, MULE, and HectH9, is a HECT (homology to E6-APC terminus)-domain E3 ubiquitin ligase originally identified as a binding partner of the tumor suppressor ARF, as well as a direct negative regulator of the tumor suppressor p53. To further elucidate the in vivo role of Huwe1, we generated a liver-specific Huwe1 (Huwe1LKO) knockout mouse model. Surprisingly, liver-specific knockout of Huwe1 protected mice from the development of age-induced hepatic steatosis. To elucidate the mechanism underlying this phenotype, mass spectrometry analysis was performed on liver tissues from 1-year-old Huwe1LKO and Huwe1WT mice.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:25:37.109.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	William Feng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-11 14:41:55	ID requested
1	2024-01-26 06:42:33	announced
⏵ 2	2024-10-22 06:25:37	announced	2024-10-22: Updated project metadata.

10.1016/j.isci.2023.108405;

Feng WW, Bang S, Takacs EM, Day C, Crawford KJ, Al-Sheyab R, Almufarrej DB, Wells W, Ilchenko S, Kasumov T, Kon N, Novak CM, Gu W, Kurokawa M, loss protects mice from non-alcoholic fatty liver disease through lipid metabolic rewiring. iScience, 26(12):108405(2023) [pubmed]

submitter keyword: NAFLD, RXR, PPAR, Steatosis

Manabu Kurokawa
contact affiliation	Department of Biological Sciences, Kent State University, USA
contact email	mkurokaw@kent.edu
lab head
William Feng
contact affiliation	Dana-Farber Cancer Institute
contact email	william_feng@dfci.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045279

PRIDE project URI

• PRIDE
  1. PXD045279
     1. Label: PRIDE project
     2. Name: Hepatic Huwe1 loss protects mice from non-alcoholic fatty liver disease through lipid metabolic rewiring