PXD045266 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Paramecium Gtsf1 is an essential nuclear PIWI-interacting protein controlling the selective degradation of small RNAs during programmed DNA elimination |
Description | Ciliates undergo developmentally programmed genome elimination, in which small RNAs direct the removal of target DNA segments, including transposable elements. At each sexual generation, the development of the macronucleus (MAC) requires massive and reproducible elimination of a large proportion of the germline micronuclear (MIC) genome, leading to a highly streamlined somatic MAC genome. 25-nt long scnRNAs are produced from the entire germline MIC genome during meiosis, and this initial complex small RNA population is then transported to the maternal MAC, where selection of scnRNAs corresponding to germline (MIC)-specific sequences is thought to take place. Selected scnRNAs, loaded onto the PIWI protein Ptiwi09, guide the deposition of histone H3 post-translational modifications (H3K9me3 and H3K27me3) onto transposable elements in the developing macronucleus, ultimately triggering their specific elimination. How germline-specific MIC scnRNAs are selected remains to be determined. Here, we provide important mechanistic insights into the scnRNA selection pathway by identifying a Paramecium homolog of Gametocyte specific factor 1 (Gtsf1) as essential for the selective degradation of scnRNAs corresponding to retained somatic MAC sequences. Consistently, we also show that Gstf1 is exclusively localized in the maternal macronucleus, where scnRNA selection is presumed to occur, and associates with the scnRNA-binding protein Ptiwi09. Furthermore, Gtsf1 is necessary for DNA elimination and correct H3K9me3 and H3K27me3 localization in the new developing macronucleus, demonstrating that the scnRNA selection process is important for genome elimination. We propose that Gtsf1 is required for the coordinated degradation of Ptiwi09-scnRNA complexes that pair with nascent RNA transcribed from the maternal MAC genome, similarly to the mechanism suggested for microRNA target-directed degradation in metazoans. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_05:48:34.512.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD045266 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Agata Malinowska |
SpeciesList | scientific name: Paramecium triaurelia; NCBI TaxID: 44031; |
ModificationList | methylthiolated residue; monohydroxylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-11 07:00:27 | ID requested | |
⏵ 1 | 2024-10-17 05:48:34 | announced | |
Publication List
Keyword List
submitter keyword: Piwi, sRNA,GTSF1, Paramecium |
Contact List
Jacek Nowak |
contact affiliation | Laboratory of Non-coding RNA and Genome Rearrangements, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland |
contact email | jknowak@ibb.waw.pl |
lab head | |
Agata Malinowska |
contact affiliation | IBB PAS |
contact email | esme@ibb.waw.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045266
- Label: PRIDE project
- Name: Paramecium Gtsf1 is an essential nuclear PIWI-interacting protein controlling the selective degradation of small RNAs during programmed DNA elimination