PXD045260

PXD045260 is an original dataset announced via ProteomeXchange.

Title	A phage nucleus-associated RNA-binding protein is required for jumbo phage infection
Description	Large-genome bacteriophages (jumbo phages) of the Chimalliviriadae family assemble a nucleus-like compartment bounded by a protein shell that protects the replicating phage genome from host-encoded restriction enzymes and CRISPR/Cas nucleases. While the nuclear shell provides broad protection against host nucleases, it necessitates transport of mRNA out of the nucleus-like compartment for translation by host ribosomes, and transport of specific proteins into the nucleus-like compartment to support DNA replication and mRNA transcription. Here we identify a conserved phage nuclear shell-associated protein that we term Chimallin C (ChmC), which adopts a nucleic acid-binding fold, binds RNA with high affinity in vitro, and binds phage mRNAs in infected cells. ChmC also forms phase-separated condensates with RNA in vitro. Targeted knockdown of ChmC using mRNA-targeting dCas13d halts infections at an early stage. Taken together, our data suggest that the conserved ChmC protein acts as a chaperone for phage mRNAs, potentially stabilizing these mRNAs and driving their translocation through the nuclear shell to promote translation and infection progression.
HostingRepository	PRIDE
AnnounceDate	2024-03-25
AnnouncementXML	Submission_2024-03-25_15:46:13.376.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045260
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eray Enustun
SpeciesList	scientific name: Pseudomonas phage PhiPA3; NCBI TaxID: 998086; scientific name: Escherichia phage vB_EcoM_Goslar; NCBI TaxID: 2502409;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos; TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-11 03:25:44	ID requested
⏵ 1	2024-03-25 15:46:13	announced

10.1093/NAR/GKAE216;

10.6019/PXD045260;

submitter keyword: Chimallin, mRNA transport, Whirly domain,Jumbo phage

Kevin D.
contact affiliation	Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA
contact email	kcorbett@ucsd.edu
lab head
Eray Enustun
contact affiliation	UCSD
contact email	eenustun@ucsd.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045260
     1. Label: PRIDE project
     2. Name: A phage nucleus-associated RNA-binding protein is required for jumbo phage infection