PXD045253

PXD045253 is an original dataset announced via ProteomeXchange.

Title	Pseudomonas putida as saviour for troubled Synechococcus elongatus in a synthetic co-culture – Interaction studies based on a multi-OMICs approach
Description	In their natural environments, microbes rarely exist in isolation; instead, they thrive in consortia where diverse interactions take place. In this study, a defined synthetic co-culture of the cyanobacterium S. elongatus cscB, which feeds the heterotrophic P. putida cscRABY with sucrose, was investigated to identify potential interactions. In initial experiments, a remarkable growth-promoting effect brought about by the presence of the heterotrophic partner on the cyanobacterium was observed, leading to a growth rate increase of up to 80% and increased photosynthetic capacity. Vice versa, the cyanobacterium had a neutral effect on P. putida cscRABY, highlighting the resilience against stress of Pseudomonads and their potential as co-culture partners. A suitable reference process reinforcing the growth-promoting effect was established in a parallel operating photobioreactor system, which sets the basis for the analysis of the co-culture at transcriptional, proteomic, and metabolomic level. This multi-OMICs approach revealed several moderate changes, including alterations in the metabolism, transportation, and stress response in both microbes, indicating multi-level interactions. These findings provide valuable insights into the complex dynamics within the co-culture system, suggesting the exchange of further molecules beyond the unidirectional feeding with sucrose.
HostingRepository	iProX
AnnounceDate	2023-09-10
AnnouncementXML	Submission_2024-05-22_19:21:54.943.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mengxun Shi
SpeciesList	scientific name: Pseudomonas putida; NCBI TaxID: 303; scientific name: Synechococcus elongatus PCC 7942 = FACHB-805; NCBI TaxID: 1140;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-11 00:34:20	ID requested
1	2023-09-11 00:37:29	announced
⏵ 2	2024-05-22 19:21:55	announced	2024-05-23: Update information.

Kratzl F, Urban M, Pandhal J, Shi M, Meng C, Kleigrewe K, Kremling A, Pfl, ü, ger-Grau K, Pseudomonas putida as saviour for troubled Synechococcus elongatus in a synthetic co-culture - interaction studies based on a multi-OMICs approach. Commun Biol, 7(1):452(2024) [pubmed]

submitter keyword: Pseudomonas putida, Synechococcus elongatus, co-cultultivation, synthetic consortia, proteomics

Jagroop Pandhal
contact affiliation	The University of Sheffield
contact email	j.pandhal@sheffield.ac.uk
lab head
Mengxun Shi
contact affiliation	The University of Sheffield
contact email	mengxunshi@hotmail.com
dataset submitter

iProX dataset URI