PXD045252

PXD045252 is an original dataset announced via ProteomeXchange.

Title	Interacting Partners of CHIKV nsP3 in Human Macrophages
Description	Chikungunya virus (CHIKV), an alphavirus transmitted by mosquitoes, has instigated several epidemics in recent years, sparking intensive efforts to understand its biology. Despite progress, the understanding of CHIKV’s molecular interactions with host cell constituents, especially in susceptible cells such as macrophages remain limited. We used mass spectrometry platform to characterize the interactions between CHIKV-nsP3, a viral nonstructural protein, and proteins in human THP-1 macrophage cells. Our findings revealed 196 high-confidence interactions primarily involving nsP3. Further the sub-cellular localization, pathways these interacting proteins might be involved in were deduced using computational methods. The interacting partners were further incorporated into a comprehensive host virus interaction network derived from extensive literature on alphavirus-host interactions. Collectively, this study offers the first interaction map between CHIKV nsP3 protein and THP-1 cells, illuminating new probable roles of host cell proteins in CHIKV’s replication cycle.
HostingRepository	PRIDE
AnnounceDate	2024-03-06
AnnouncementXML	Submission_2024-03-05_22:50:38.936.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sujatha Sunil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-11 00:29:57	ID requested
⏵ 1	2024-03-05 22:50:39	announced

10.3389/FVIRO.2024.1310161;

submitter keyword: virus host interaction, macrophages, nonstructural protein 3, proteome,CHIKV

Dr. Sujatha
contact affiliation	Group Leader, Vector Borne Diseases International Centre for Genetic Engineering and Biotechnology Aruna Asaf Ali Marg 110 067 New Delhi, India
contact email	sujatha@icgeb.res.in
lab head
Sujatha Sunil
contact affiliation	International Centre for Genetic Engineering and Biotechnology
contact email	sujatha@icgeb.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045252
     1. Label: PRIDE project
     2. Name: Interacting Partners of CHIKV nsP3 in Human Macrophages