PXD045235

PXD045235 is an original dataset announced via ProteomeXchange.

Title	Optimized isolation of protein on chromatin (iPOC) strategy reveals the dynamics of chromatin composition upon inhibition of PI3K/AKT/mTOR signaling
Description	Signaling pathways play critical roles in executing and controlling important biological processes within cells. The dysregulation of signaling pathways modulates chromatin modifications to regulate the epigenome, thereby contributing to tumorigenesis and metastasis Despite their cancer-related importance, current studies are limited to the investigation of the signaling cascade and how to shut this down in a cancer context. Yet the ultimate effect of PI3K/AKT/mTOR activation cascades on chromatin composition have not been previously investigated. To unravel DBP function upon different pathway inhibitors, in this study, we 1) optimized the iPOC strategy, 2) assessed both the specificity and sensitivity of the approach, and 3) performed comprehensive quantitative chromatin proteomic analysis to identify the dynamics of DNA-bound proteome upon treatment with PI3K, AKT or mTOR inhibitors.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:44:38.258.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huiyu Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-08 17:50:07	ID requested
⏵ 1	2025-05-06 13:44:39	announced

Wang H, Syed AA, Krijgsveld J, Sigismondo G, Isolation of Proteins on Chromatin Reveals Signaling Pathway-Dependent Alterations in the DNA-Bound Proteome. Mol Cell Proteomics, 24(3):100908(2025) [pubmed]

10.1016/j.mcpro.2025.100908;

submitter keyword: PI3K/AKT/mTOR, LC/MS-MS, DNA binding proteomics

Jeroen Krijgsveld
contact affiliation	German cancer research center, Proteomics of stem cells and cancer, B230
contact email	j.krijgsveld@dkfz-heidelberg.de
lab head
Huiyu Wang
contact affiliation	18272583259
contact email	lizlemon@sjtu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD045235

PRIDE project URI

• PRIDE
  1. PXD045235
     1. Label: PRIDE project
     2. Name: Optimized isolation of protein on chromatin (iPOC) strategy reveals the dynamics of chromatin composition upon inhibition of PI3K/AKT/mTOR signaling