To identify the substrates of RNF114 in the context of PARylation-mediated DDR, we performed an IP-MS (immunoprecipitation coupled with MS) experiment, using cells treated with either H2O2 or H2O2+Talazoparib. We identified a large number of PARP1 peptides from the RNF114 immunoprecipitants only in H2O2-treated cells.