PXD045228

PXD045228 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling of MOLM-13 cells treated with proposed CK1α degraders
Description	Targeted Protein Degradation (TPD) encompasses two approaches that generate small molecules with similar proteasome-dependent mechanisms of action: Proteolysis-Targeting Chimeras (PROTACs) and Molecular Glues (MGs). Our goal is to develop novel MG approaches that exploit the E3 ubiquitin ligase Cereblon (CRBN) to degrade key oncogenic drivers in the leading causes of childhood cancer death: acute myeloid leukemia (AML). This project will identify new vulnerabilities and compounds that degrade hitherto undruggable transcription factors in high-risk acute leukemia (AL) and medulloblastoma (MB). Based on our preliminary data, we hypothesize that each of these compounds (SJ001010039, SJ001010040, SJ001010041, SJ001010047, SJ001010050) degrades CK1α in this cell line and may degrade other proteins. Our aims are to confirm that CK1α is degraded by treatment with each of these compounds and to identify any additional proteins that are targeted for degradation by these compounds.
HostingRepository	PRIDE
AnnounceDate	2023-12-02
AnnouncementXML	Submission_2023-12-01_17:37:26.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yingxue Fu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-08 09:51:14	ID requested
⏵ 1	2023-12-01 17:37:27	announced

Dataset with its publication pending

submitter keyword: Acute Leukemia,Targeted Protein Degradation, TMT, Molecular Glues, LC-MS/MS

Junmin Peng
contact affiliation	Department of Structural Biology and Developmental Neurobiology, Center for Proteomics and Metabolomics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA
contact email	junmin.peng@stjude.org
lab head
Yingxue Fu
contact affiliation	St. Jude Children's Research Hospital
contact email	yingxue.fu@stjude.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD045228

PRIDE project URI

• PRIDE
  1. PXD045228
     1. Label: PRIDE project
     2. Name: Proteome profiling of MOLM-13 cells treated with proposed CK1α degraders