PXD045227 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DOPA formation on tyrosine Y150 of Ribonucleotide reductase QSK R2e variant from Gardnerella vaginalis. |
Description | With a crystal structure and mass spectrometry on recombinantly expressed and purified Ribonucleotide reductase QSK R2e variant from Gardnerella vaginalis we show that this protein has a modified DOPA (3,4-Dihydroxy-L-phenylalanine) in the active site analogous to what was observed for the VPK variant. Moreover, we show that this DOPA formation is facilitated by the coexpression of partner proteins NrdI and surprisingly also NrdH and R1 from the same organism. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:02:52.071.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rui Branca |
SpeciesList | scientific name: Gardnerella vaginalis; NCBI TaxID: 2702; |
ModificationList | L-3',4'-dihydroxyphenylalanine |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-08 08:44:01 | ID requested | |
1 | 2024-10-07 13:50:08 | announced | |
⏵ 2 | 2024-10-22 06:02:52 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: QExactive HF, 3, PTM, Gardnerella vaginalis,4-dihydroxyphenylalanine, tyrosine,Ribonuclotide reductase, DOPA |
Contact List
Janne Lehtio |
contact affiliation | Dept. Oncology-Pathology, SciLifeLab, Karolinska Institutet, Sweden |
contact email | janne.lehtio@ki.se |
lab head | |
Rui Branca |
contact affiliation | Clinical Proteomics Unit, Dep. of Oncology-Pathology |
contact email | rui.mamede-branca@ki.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD045227 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045227
- Label: PRIDE project
- Name: DOPA formation on tyrosine Y150 of Ribonucleotide reductase QSK R2e variant from Gardnerella vaginalis.