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PXD045227

PXD045227 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDOPA formation on tyrosine Y150 of Ribonucleotide reductase QSK R2e variant from Gardnerella vaginalis.
DescriptionWith a crystal structure and mass spectrometry on recombinantly expressed and purified Ribonucleotide reductase QSK R2e variant from Gardnerella vaginalis we show that this protein has a modified DOPA (3,4-Dihydroxy-L-phenylalanine) in the active site analogous to what was observed for the VPK variant. Moreover, we show that this DOPA formation is facilitated by the coexpression of partner proteins NrdI and surprisingly also NrdH and R1 from the same organism.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:02:52.071.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRui Branca
SpeciesList scientific name: Gardnerella vaginalis; NCBI TaxID: 2702;
ModificationListL-3',4'-dihydroxyphenylalanine
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-08 08:44:01ID requested
12024-10-07 13:50:08announced
22024-10-22 06:02:52announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: QExactive HF, 3, PTM, Gardnerella vaginalis,4-dihydroxyphenylalanine, tyrosine,Ribonuclotide reductase, DOPA
Contact List
Janne Lehtio
contact affiliationDept. Oncology-Pathology, SciLifeLab, Karolinska Institutet, Sweden
contact emailjanne.lehtio@ki.se
lab head
Rui Branca
contact affiliationClinical Proteomics Unit, Dep. of Oncology-Pathology
contact emailrui.mamede-branca@ki.se
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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