PXD045193

PXD045193 is an original dataset announced via ProteomeXchange.

Title	Optimising a self-assembling peptide hydrogel as a Matrigel alternative for 3-dimensional mammary epithelial cell culture
Description	Three-dimensional (3D) organoid models have been instrumental in understanding molecular mechanisms responsible for many cellular processes and diseases. However, established organic biomaterial scaffolds used for 3D hydrogel cultures, such as Matrigel, are biochemically complex and display significant batch variability, limiting reproducibility in experiments. Recently, there has been significant progress in the development of synthetic hydrogels for in vitro cell culture that are reproducible, mechanically tuneable, and biocompatible. Self-assembling peptide hydrogels (SAPHs) are synthetic biomaterials that can be engineered to be compatible with 3D cell culture. Here we investigate the ability of PeptiGel® SAPHs to model mammary epithelial cell (MEC) microenvironment in vitro. The positively charged PeptiGel®Alpha4 supported MEC viability, but did not promote formation of polarised acini. Modifying the stiffness of PeptiGel®Alpha4 stimulated changes in MEC viability and changes in protein expression associated with altered MEC function, but did not fully recapitulate the morphologies of MECs grown in Matrigel. To supply the appropriate biochemical signals for MEC organoids, we supplemented PeptiGels® with laminin. Laminin was found to require negatively charged PetiGel®Alpha7 for functionality, but was then able to provide appropriate signals for correct MEC polarisation and expression of characteristic proteins. Thus, optimisation of SAPH composition and mechanics allows tuning to support tissue-specific organoids.
HostingRepository	PRIDE
AnnounceDate	2024-05-22
AnnouncementXML	Submission_2024-05-22_06:27:07.859.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Hoyle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-07 06:59:13	ID requested
⏵ 1	2024-05-22 06:27:08	announced

10.1016/j.bioadv.2024.213847;

Lingard E, Dong S, Hoyle A, Appleton E, Hales A, Skaria E, Lawless C, Taylor-Hearn I, Saadati S, Chu Q, Miller AF, Domingos M, Saiani A, Swift J, Gilmore AP, Optimising a self-assembling peptide hydrogel as a Matrigel alternative for 3-dimensional mammary epithelial cell culture. Biomater Adv, 160():213847(2024) [pubmed]

submitter keyword: mammary epithelial cell,3D, laminin, organoid

Andrew Gilmore
contact affiliation	Division of Cancer Sciences, University of Manchester
contact email	andrew.gilmore@manchester.ac.uk
lab head
Anna Hoyle
contact affiliation	University of Manchester
contact email	anna.hoyle@postgrad.manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045193
     1. Label: PRIDE project
     2. Name: Optimising a self-assembling peptide hydrogel as a Matrigel alternative for 3-dimensional mammary epithelial cell culture