PXD045191

PXD045191 is an original dataset announced via ProteomeXchange.

Title	Direct recruitment of Mis18 to interphase spindle poles promotes CENP-A chromatin assembly.
Description	CENP-A chromatin specifies mammalian centromere identity, and its chaperone HJURP replenishes CENP-A when recruited by the Mis18 complex (Mis18C) via M18BP/KNL2 to CENP-C at kinetochores during interphase. However, the Mis18C recruitment mechanism remains unresolved in species lacking M18BP1, such as fission yeast. Fission yeast centromeres cluster at G2 spindle pole bodies (SPBs) when CENP-ACnp1 is replenished and where Mis18C also localizes. We show that SPBs play an unexpected role in concentrating Mis18C near centromeres through the recruitment of Mis18 by direct binding to the major SPB LInker of Nucleoskeleton and Cytoskeleton (LINC) complex component Sad1. Mis18 recruitment by Sad1 is important for CENP-ACnp1 chromatin establishment and acts in parallel with a CENP-C-mediated Mis18C recruitment pathway to maintain centromeric CENP-ACnp1, but is independent of Sad1-mediated centromere clustering. SPBs therefore provide a non-chromosomal scaffold for both Mis18C recruitment and centromere clustering during G2. This centromere-independent Mis18-SPB recruitment provides a mechanism that governs de novo CENP-ACnp1 chromatin assembly by the proximity of appropriate sequences to SPBs and highlights how nuclear spatial organization influences centromere identity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:03:14.658.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nitobe London
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-07 05:05:30	ID requested
1	2023-09-18 01:29:12	announced
2	2023-11-14 08:54:46	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:03:15	announced	2024-10-22: Updated project metadata.

10.1016/J.CUB.2023.08.063;

submitter keyword: Mis18 complex, fission yeast,CENP-A chromatin, centromere

Robin Allshire
contact affiliation	University of Edinburgh
contact email	robin.allshire@ed.ac.uk
lab head
Nitobe London
contact affiliation	University of Edinburgh
contact email	nitobelondon@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD045191

PRIDE project URI

• PRIDE
  1. PXD045191
     1. Label: PRIDE project
     2. Name: Direct recruitment of Mis18 to interphase spindle poles promotes CENP-A chromatin assembly.