PXD045164

PXD045164 is an original dataset announced via ProteomeXchange.

Title	Protein Kinase C activation regulates NDRG1 expression
Description	N-myc downstream regulated gene 1 (NDRG1) is a member of the NDRG family of intracellular proteins, and plays a central role in a wide range of biological processes including stress response, differentiation, and maintenance of the myelin sheath. The overexpression of NDRG1 is an indicator of poor prognosis in various pathological conditions. Here, we found that NDRG1 is an independent prognostic marker of poor outcome in breast cancer (BC). NDRG1 expression is regulated by a variety of molecular mechanisms, including transcriptional and post-translational control. In the present study, CRISPR-based inactivation of NDRG1 allowed us to demonstrate that this protein is required for breast cancer cell invasion, without affecting viability. We observed that different acute stress conditions converge on protein kinase C (PKC) activation driving enhanced NDRG1 expression through a signaling pathway that involves ROCK/AMPK/Akt kinases. This newly discovered mechanism was specific for NDRG1 as the expression of other NDRG members was not affected. Together, our results suggest that pathophysiological PKC-mediated activation of NDRG1 may be a response mechanism to metabolic stress and anticancer agents.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_12:03:09.850.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Soulaimane Aboulouard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-06 10:54:28	ID requested
⏵ 1	2025-05-06 12:03:10	announced

10.1186/s13578-024-01336-z;

Saponaro C, Damato M, Stanca E, Aboulouard S, Zito FA, De Summa S, Traversa D, Schirosi L, Bravaccini S, Pirini F, Fonzi E, Tebaldi M, Puccetti M, Gaballo A, Pantalone L, Ronci M, Magnani L, Sergi D, Tinelli A, Tacconi S, Siculella L, Giudetti AM, Fournier I, Salzet M, Trerotola M, Vergara D, Unraveling the protein kinase C/NDRG1 signaling network in breast cancer. Cell Biosci, 14(1):156(2024) [pubmed]

submitter keyword: TNBC, Breast Cancer, PKC,NDRG1

Michel Salzet
contact affiliation	Laboratoire PRISM Universite de Lille
contact email	michel.salzet@univ-lille.fr
lab head
Soulaimane Aboulouard
contact affiliation	PRISM
contact email	soulaimane.aboulouard@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD045164

PRIDE project URI

• PRIDE
  1. PXD045164
     1. Label: PRIDE project
     2. Name: Protein Kinase C activation regulates NDRG1 expression