PXD045162

PXD045162 is an original dataset announced via ProteomeXchange.

Title	Multi-Level Analysis of Gut Microbiome Extracellular Vesicles-Host Interaction Reveals a Connection to Gut-Brain Axis Signaling
Description	Microbiota-released extracellular vesicles (MEVs) have emerged as a key player in intercellular signaling. However, their involvement in the gut-brain axis has been poorly investigated. In this study, we aimed to investigate the cargo capacity of MEVs for bioactive metabolites and their interactions with the host. Metabolomics analysis identified various neuro-related compounds encapsulated within the 28 MEVs, such as arachidonyl-dopamine, gabapentin, glutamate, and N-acylethanolamines. 29 Metaproteomics unveiled an enrichment of enzymes involved in neuronal metabolism, primarily in the glutamine/glutamate/GABA pathway. The detected neuro-related proteins and metabolites were correlated with Bacteroides spp. A GABA-producing Bacteroides isolate, B. finegoldii, released EVs with a high GABA content (4 µM) as opposed to a low GABA-producing isolate, Phocaeicola massiliensis. MEVs exhibited a dose-dependent paracellular transport and were endocytosed by Caco-2 and hCMEC/D3 cells. RNA-Seq analyses showed that MEVs stimulate several immune pathways while suppressing cell apoptosis process. The in vivo biodistribution confirmed the presence of MEVs in the brain, liver, stomach, and spleen. Overall, our results highlight the ability of MEVs to cross the intestinal and blood-brain barriers to deliver their cargoes to distant organs, including the brain, where it may modulate the organ functionalities. MEVs could be an integral part of microbiome-host communications, with potential implication for the gut-brain axis.
HostingRepository	PRIDE
AnnounceDate	2024-11-05
AnnouncementXML	Submission_2024-11-05_13:42:27.857.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Basit Yousuf
SpeciesList	scientific name: Bacteroides finegoldii; NCBI TaxID: 338188; scientific name: Phocaeicola massiliensis; NCBI TaxID: 204516;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-06 09:28:53	ID requested
⏵ 1	2024-11-05 13:42:28	announced

10.1128/SPECTRUM.01368-24;

submitter keyword: gut microbiome

extracellular vesicles

neuroactive metabolites

gut-brain axis

Bacteroides

γ-aminobutyric acid (GABA)

Riadh Hammami
contact affiliation	NuGut Research Platform, School of Nutrition Sciences, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada
contact email	riadh.hammami@uottawa.ca
lab head
Basit Yousuf
contact affiliation	University of Ottawa
contact email	basucsmcri@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD045162

PRIDE project URI

• PRIDE
  1. PXD045162
     1. Label: PRIDE project
     2. Name: Multi-Level Analysis of Gut Microbiome Extracellular Vesicles-Host Interaction Reveals a Connection to Gut-Brain Axis Signaling