PXD045152

PXD045152 is an original dataset announced via ProteomeXchange.

Title	A revised model for the Type A glycan biosynthetic pathway in Clostridioides difficile strain 630Δerm based on quantitative proteomics of cd0241-cd0244 mutant strains
Description	The bacterial flagellum is involved in a variety of processes including motility, adherence and immunomodulation. In the Clostridioides difficile strain 630Δerm, the main filamentous component, FliC, is post-translationally modified with an O-linked Type A glycan structure. This modification is essential for flagellar function since motility is seriously impaired in gene mutants with improper biosynthesis of the Type A glycan. The cd0240-cd0244 gene cluster encodes the Type A structure, but the role of each gene, and the corresponding enzymatic activity, has not been fully elucidated. Using quantitative mass spectrometry-based proteomics analyses, we determined the relative abundance of the observed glycan variations of the Type A structure in cd0241, cd0242, cd0243 and cd0244 mutant strains. Our data not only confirm the importance of CD0241, CD0242 and CD0243, but, in contrast to previous data, also show that CD0244 is essential for the biosynthesis of the Type A modification. Combined with additional bio-informatic analyses, we propose a revised model for Type A glycan biosynthesis.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:45:45.386.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bart Claushuis
SpeciesList	scientific name: Peptoclostridium difficile (strain 630) (Clostridium difficile); NCBI TaxID: 272563;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-06 05:00:16	ID requested
⏵ 1	2024-01-26 06:45:46	announced

10.1021/acsinfecdis.3c00485;

Claushuis B, de Ru AH, Rotman SA, van Veelen PA, Dawson LF, Wren BW, Corver J, Smits WK, Hensbergen PJ, Mutant Strains. ACS Infect Dis, 9(12):2665-2674(2023) [pubmed]

submitter keyword: biosynthesis, proteomics, mass spectrometry, enzyme,Glycation, flagella

Peter Antonius van Veelen
contact affiliation	Center for Proteomics and Metabolomics, LUMC, Leiden, The Netherlands
contact email	p.a.van_veelen@lumc.nl
lab head
Bart Claushuis
contact affiliation	Leiden University Medical Center
contact email	b.claushuis@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045152

PRIDE project URI

• PRIDE
  1. PXD045152
     1. Label: PRIDE project
     2. Name: A revised model for the Type A glycan biosynthetic pathway in Clostridioides difficile strain 630Δerm based on quantitative proteomics of cd0241-cd0244 mutant strains