PXD045145 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Highly contractile 3D tissue engineered skeletal muscles from human iPSCs reveal similarities with primary myoblast-derived tissues |
Description | Skeletal muscle research is transitioning towards 3D tissue engineered in vitro models reproducing muscle’s native architecture and supporting measurement of functionality. Human induced pluripotent stem cells (hiPSCs) offer high yields of cells for differentiation. It has been difficult to differentiate high quality, pure 3D muscle tissues from hiPSCs that show contractile properties comparable to primary myoblast-derived tissues. Here, we present a transgene-free method for the generation of purified, expandable myogenic progenitors (MPs) from hiPSCs grown under feeder-free conditions. We defined a protocol with optimal hydrogel and medium conditions that allowed production of highly contractile 3D tissue engineered skeletal muscles with forces similar to primary myoblast-derived tissues. Gene expression and proteomic analysis between hiPSC-derived and primary myoblast-derived 3D tissues revealed a similar expression profile of proteins involved in myogenic differentiation and sarcomere function. The protocol should be generally applicable for the study of personalized human skeletal muscle tissue in health and disease. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:04:32.138.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Theresa Bock |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Eclipse; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-06 02:00:29 | ID requested | |
1 | 2023-10-02 08:10:20 | announced | |
2 | 2023-11-14 09:05:43 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 06:04:32 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.stemcr.2023.08.014; |
van der Wal E, Iuliano A, In 't Groen SLM, Bholasing AP, Priesmann D, Sharma P, den Hamer B, Saggiomo V, Kr, ü, ger M, Pijnappel WWMP, de Greef JC, Highly contractile 3D tissue engineered skeletal muscles from human iPSCs reveal similarities with primary myoblast-derived tissues. Stem Cell Reports, 18(10):1954-1971(2023) [pubmed] |
Keyword List
submitter keyword: myongeic differentiation, hiPSC,3D tissue |
Contact List
W. W. M. Pim Pijnappel |
contact affiliation | Department of Clinical Genetics, Erasmus University Medical Center, 3015 GE Rotterdam, Netherlands Department of Pediatrics, Erasmus University Medical Center, 3015 GE Rotterdam, Netherlands Center for Lysosomal and Metabolic Diseases, Erasmus University Medical Center, 3015 GE Rotterdam, Netherlands |
contact email | w.pijnappel@erasmusmc.nl |
lab head | |
Theresa Bock |
contact affiliation | CECAD Research Center, University of Cologne |
contact email | tbock2@uni-koeln.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD045145 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045145
- Label: PRIDE project
- Name: Highly contractile 3D tissue engineered skeletal muscles from human iPSCs reveal similarities with primary myoblast-derived tissues