PXD045140

PXD045140 is an original dataset announced via ProteomeXchange.

Title	Proteomics for Roseburia intestinalis and Parabacteroides merdae exposed to isosteviol and duloxetine.
Description	The objective of this project was to identify changes in protein expression in R. intestinalis and P. merdae as a response to the treatment with the antidepressant duloxetine, the sweetener isosteviol and a combination of both. 18 samples before and after treatment were collected for each of the two species under anaerobic conditions. The species were inoculated from frozen stock cultures, three replicates each, into mGAM medium and passaged twice over night. Passage two was inoculated into 60 ml (120 ml for R. intestinalis) medium and pre-exposure0 samples were collected directly hereafter (three per species, one sample per replicate). The second pre-exposure samples were collected upon reaching exponential growth, directly prior to splitting each replicate into four equal sized batches, one per condition: Mock (DMSO, 0.2 %), 50 µM duloxetine, 50 µM isosteviol and a combination of both, 50 µM each. This results in 12 treatment samples, three per treatment, whereas each of the replicates can be traced back to one preculture. Bacteria were grown in the presence of the compounds at 37˚C for four hours, before final sample collection. Pre-exposure samples were collected as controls to monitor changes in protein expression in different growth stages.
HostingRepository	PRIDE
AnnounceDate	2026-04-30
AnnouncementXML	Submission_2026-04-30_07:04:45.612.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sonja Blasche
SpeciesList	scientific name: Parabacteroides merdae; NCBI TaxID: NEWT:46503; scientific name: Roseburia intestinalis; NCBI TaxID: NEWT:166486;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-06 01:12:48	ID requested
⏵ 1	2026-04-30 07:04:46	announced

Dataset with its publication pending

submitter keyword: proteomics, antidepressant duloxetine,Gut bacteria, sweetener isosteviol

Kiran R Patil
contact affiliation	Medical Research Council Toxicology Unit, University of Cambridge, Cambridge, UK
contact email	kp533@cam.ac.uk
lab head
Sonja Blasche
contact affiliation	MRC Toxicology, Uni of Cambridge
contact email	sb2487@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD045140

PRIDE project URI

• PRIDE
  1. PXD045140
     1. Label: PRIDE project
     2. Name: Proteomics for Roseburia intestinalis and Parabacteroides merdae exposed to isosteviol and duloxetine.