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PXD045122

PXD045122 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCdc48’s interactions play a role in the cellular oxidative response in an aging-dependent manner
DescriptionThe cellular redox status has long been linked to different cellular functions, including protein quality control (PQC) and aging. The prominent molecular machine Cdc48 (p97/VCP in mammals) is one of the key players involved in PQC, shuttling misfolded proteins to degradation. Through a range of N-terminal interactions, Cdc48 recognizes ubiquitin-tagged proteins targeted for proteasomal degradation. Here, we show that yeast Cdc48 is a redox-switch protein, with oxidation of a thiol-switch in the conserved Cys115. Using redox mass spectrometry, we characterize the oxidation status of Cdc48’s cysteine residues in the shift from the early- to late-stationary stages. Cdc48’s interactome undergoes a proteomic-scale rearrangement following this oxidation and leads to an increased association with antioxidants. Moreover, we found that the oxidation status of Cys115 mediates the activity of Cdc48, its interaction with canonical cofactors such as Shp1, and subsequent involvement in maintaining protein homeostasis during chronological aging. This work not only points to a new thiol switch protein in the protein degradation pathway, but also defines new roles for Cdc48 in chronological aging.
HostingRepositoryPRIDE
AnnounceDate2025-05-28
AnnouncementXMLSubmission_2025-05-28_12:51:46.614.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMeytal Radzinski
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListN-ethylmaleimide derivatized cysteine; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-05 17:51:19ID requested
12025-05-28 12:51:47announced
Publication List
Radzinski M, Oppenheim T, Yogev O, Levy A, Naomi MB, Kacen A, Merbl Y, Ravid T, Reichmann D, Cdc48 plays a crucial role in redox homeostasis through dynamic reshaping of its interactome during early stationary phase. Redox Biol, 84():103651(2025) [pubmed]
10.1016/j.redox.2025.103651;
Keyword List
submitter keyword: redox,yeast, aging
Contact List
Dana Reichmann
contact affiliationDept. of Biological Chemistry,The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem,Givat Ram Campus, Jerusalem, 91904, Israel
contact emaildanare@mail.huji.ac.il
lab head
Meytal Radzinski
contact affiliationHebrew University of Jerusalem
contact emailmeytal.radzinski@mail.huji.ac.il
dataset submitter
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