PXD045112

PXD045112 is an original dataset announced via ProteomeXchange.

Title	Phanerochaete chrysosporium RP78 v.4 LC-MSMS
Description	The influence of substrate composition on extracellular protein production was evaluated in media containing glucose, lodgepole pine or aspen as carbon sources. Common to all media, basal salts contained, per liter, 2g NH4NO3, 2g KH2, 0.5 g MgSO4·7H2O, 0.1 g CaCl2·2H2O, 1 mg thiamine hydrochloride and 1 mL trace element solution (Teknova, T1001). Aspen and pine were added to 5 g per liter, while the glucose cultures received 2.5 g per liter. All cultures contained 100 mL media in 500 ml Erlenmeyer flasks and, following inoculation with macerated mycelium, incubated on a rotary shaker (100 RPM) at 24°C. Aspen (Populus grandidentata) and lodgepole pine (Pinus contorta) were Wiley milled and sieved to one mm mesh prior to inoculation. Triplicate cultures were harvested after 5 and 10 days.
HostingRepository	PRIDE
AnnounceDate	2023-09-15
AnnouncementXML	Submission_2023-09-15_10:35:35.136.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045112
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	GregSabat
SpeciesList	scientific name: Phanerochaete chrysosporium RP-78; NCBI TaxID: 273507;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-05 08:47:55	ID requested
⏵ 1	2023-09-15 10:35:35	announced

10.6019/PXD045112;

submitter keyword: LC-MSMS, lignocellulose,Phanerochaete chrysosporium

Dr.Daniel
contact affiliation	Forest Products Laboratory, Institute for Microbial and Biochemical Sciences, USDA
contact email	daniel.cullen@wisc.edu
lab head
GregSabat
contact affiliation	University of Wisconsin
contact email	gsabat@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045112
     1. Label: PRIDE project
     2. Name: Phanerochaete chrysosporium RP78 v.4 LC-MSMS