PXD045089

PXD045089 is an original dataset announced via ProteomeXchange.

Title	A quick guideline for PRM method development applied to target proteomics in biological fluids
Description	Combining in-silico analysis, serum high abundant protein depletion, and DDA and PRM acquisitions, we established a simple workflow to select PRM high response peptides for protein quantification in serum. The workflow enables evaluating peptide intensity, MS2 match with the spectral library, peptide stability, and linearity of response to different injected masses. This workflow was successfully applied to twelve human complement system proteins. Besides, we employed synthetic labelled peptides to compensate inter LC-MS run variability, improving the quantification. We could demonstrate this method is useful and applicable for quantification of diverse targets with time optimization and accuracy insurance by quantitative proteomics technology.
HostingRepository	PRIDE
AnnounceDate	2024-02-05
AnnouncementXML	Submission_2024-02-05_06:25:10.755.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michel Batista
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Exploris 120

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-04 17:54:00	ID requested
⏵ 1	2024-02-05 06:25:11	announced

Dataset with its publication pending

submitter keyword: complement system,PRM, human serum, quantitative proteomics, LC-MS/MS

Fabiano Borges Figueiredo
contact affiliation	Laboratory of Cell Biology, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba, Parana, Brazil
contact email	fabiano.figueiredo@fiocruz.br
lab head
Michel Batista
contact affiliation	Fiocruz
contact email	batista.mic@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD045089

PRIDE project URI

• PRIDE
  1. PXD045089
     1. Label: PRIDE project
     2. Name: A quick guideline for PRM method development applied to target proteomics in biological fluids