PXD045084

PXD045084 is an original dataset announced via ProteomeXchange.

Title	Toxicological Effects and Underlying Mechanisms of Chlorination–Derived Metformin Byproducts in Escherichia coli
Description	Chlorination–derived byproducts of the emerging contaminant metformin, such as 3,3-CDTA and NCDC, occurring in global waters are toxic to biota, be it from bacteria to mice. However, the underlying toxicological mechanisms remain unknown. Here, we explored the toxicological effects and underlying molecular mechanisms of 3,3-CDTA and NCDC at milligram levels using Escherichia coli as a model organism. Compared with metformin (>300 mg/L), 3,3-CDTA and NCDC exerted stronger toxicity to E. coli with the 4-h IC50 of 2.97 mg/L and 75.7 mg/L, respectively. Both byproducts disrupted E. coli cellular structures and components, decreased membrane potential and adenosine triphosphate (ATP) biosynthesis, and produced excessive reactive oxidative species (ROSs), superoxide dismutase (SOD), and catalase. Proteomics analysis and molecular docking not only supported these biomarker responses in the byproduct-treated E. coli and indicated possible the damage of DNA/RNA process but also presented a novel insight into their toxicological and detoxification effects at the proteome level. Therefore, the mechanistically toxicological effects by which NCDC and 3,3-CDTA are toxic were ascribed to membrane disruption, oxidative stress, and abnormal protein expression. This study discusses the first toxicological effects of chlorination–derived metformin byproducts in E. coli and broadens understanding of the toxic effects and transformation risks of metformin throughout its entire life process.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:06:00.888.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuanzhen He
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-04 07:21:23	ID requested
1	2023-10-16 17:42:33	announced
2	2023-11-14 08:32:37	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:06:01	announced	2024-10-22: Updated project metadata.

10.1016/J.SCITOTENV.2023.167281;

submitter keyword: biomarkers,E. coli, LC-MS/MS, chlorinated byproducts

Feng Ju
contact affiliation	Enbironmental Microbiome and Biotechnology Laboratory, Key Laboratory of Coastal Environment and Resources of Zhejiang Province, School of Engineering, Westlake University, Hangzhou, Zhejiang 310030, China
contact email	jufeng@westlake.edu.cn
lab head
Yuanzhen He
contact affiliation	Westlake University
contact email	heyuanzhen@westlake.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD045084

PRIDE project URI

• PRIDE
  1. PXD045084
     1. Label: PRIDE project
     2. Name: Toxicological Effects and Underlying Mechanisms of Chlorination–Derived Metformin Byproducts in Escherichia coli