⮝ Full datasets listing

PXD045084

PXD045084 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleToxicological Effects and Underlying Mechanisms of Chlorination–Derived Metformin Byproducts in Escherichia coli
DescriptionChlorination–derived byproducts of the emerging contaminant metformin, such as 3,3-CDTA and NCDC, occurring in global waters are toxic to biota, be it from bacteria to mice. However, the underlying toxicological mechanisms remain unknown. Here, we explored the toxicological effects and underlying molecular mechanisms of 3,3-CDTA and NCDC at milligram levels using Escherichia coli as a model organism. Compared with metformin (>300 mg/L), 3,3-CDTA and NCDC exerted stronger toxicity to E. coli with the 4-h IC50 of 2.97 mg/L and 75.7 mg/L, respectively. Both byproducts disrupted E. coli cellular structures and components, decreased membrane potential and adenosine triphosphate (ATP) biosynthesis, and produced excessive reactive oxidative species (ROSs), superoxide dismutase (SOD), and catalase. Proteomics analysis and molecular docking not only supported these biomarker responses in the byproduct-treated E. coli and indicated possible the damage of DNA/RNA process but also presented a novel insight into their toxicological and detoxification effects at the proteome level. Therefore, the mechanistically toxicological effects by which NCDC and 3,3-CDTA are toxic were ascribed to membrane disruption, oxidative stress, and abnormal protein expression. This study discusses the first toxicological effects of chlorination–derived metformin byproducts in E. coli and broadens understanding of the toxic effects and transformation risks of metformin throughout its entire life process.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:06:00.888.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYuanzhen He
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-04 07:21:23ID requested
12023-10-16 17:42:33announced
22023-11-14 08:32:37announced2023-11-14: Updated project metadata.
32024-10-22 06:06:01announced2024-10-22: Updated project metadata.
Publication List
10.1016/J.SCITOTENV.2023.167281;
Keyword List
submitter keyword: biomarkers,E. coli, LC-MS/MS, chlorinated byproducts
Contact List
Feng Ju
contact affiliationEnbironmental Microbiome and Biotechnology Laboratory, Key Laboratory of Coastal Environment and Resources of Zhejiang Province, School of Engineering, Westlake University, Hangzhou, Zhejiang 310030, China
contact emailjufeng@westlake.edu.cn
lab head
Yuanzhen He
contact affiliationWestlake University
contact emailheyuanzhen@westlake.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD045084
PRIDE project URI
Repository Record List
[ + ]