PXD045082

PXD045082 is an original dataset announced via ProteomeXchange.

Title	Extracellular proteins enhance Cupriavidus pauculus nickel tolerance and cell aggregate formation
Description	Heavy metal-resistant bacteria secrete extracellular proteins (e-PNs). However, the role of e-PNs in heavy metal resistance remains elusive. Here Fourier Transform Infrared Spectroscopy implied that N-H, C = O and NH2-R played a crucial role in the adsorption and resistance of Ni2+ in the model organism Cuprividus pauculus 1490 (C. pauculus). Proteinase K treatment reduced Ni2+ resistance of C. pauculus underlining the essential role of e-PNs. Further three-dimension excitation-emission matrix fluorescence spectroscopy analysis demonstrated that tryptophan proteins as part of the e-PNs increased significantly with Ni2+ treatment. Proteomic and quantitative real-time polymerase chain reaction data indicated that major changes were induced in the metabolism of C. pauculus in response to Ni2+. Among those lipopolysaccharide biosynthesis, general secretion pathways, Ni2+-affiliated transporters and multidrug efflux play an essential role in Ni2+ resistance. Altogether the results provide a conceptual model for comprehending how e-PNs contribute to bacterial resistance and adsorption of Ni2+.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:18:14.537.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bente Siebels
SpeciesList	scientific name: Cupriavidus pauculus; NCBI TaxID: 82633;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-04 06:37:49	ID requested
1	2023-12-21 07:03:51	announced
⏵ 2	2024-10-22 06:18:14	announced	2024-10-22: Updated project metadata.

10.1016/J.BIORTECH.2023.130133;

submitter keyword: extracellular proteins

Cupriavidus pauculus

nickel resistance

secretome

efflux pumps

Wolfgang R.
contact affiliation	Department of Microbiology and Biotechnology, University of Hamburg, Hamburg, 22609, Germany
contact email	wolfgang.streit@uni-hamburg.de
lab head
Bente Siebels
contact affiliation	Section for Mass spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf
contact email	b.siebels@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD045082

PRIDE project URI

• PRIDE
  1. PXD045082
     1. Label: PRIDE project
     2. Name: Extracellular proteins enhance Cupriavidus pauculus nickel tolerance and cell aggregate formation