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PXD045077

PXD045077 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics of secretomes from Agaricus bisporus grown on 12C-lignin
DescriptionIn the A. bisporus treated lignin (12CLg+Ab; pure lignin as sole C-source) secretome, a total of 500 (putative) proteins were detected, of which 72 were carbohydrate-active enzymes (CAZymes). Furthermore, we found 27 enzymes that are active on lignin and aromatics. Among those enzymes, 23 are classified as Auxiliary Activities (AA; CAZy.org), of which eight belong to subfamily AA1_1, another eight to subfamily AA3_2, five to subfamily AA5_1, and one to subfamilies AA1_2 and AA2. Interestingly, six AA1_1 members were annotated as sensu stricto laccases. Proteins from subfamilies AA5_1 and AA3_2 can be further categorized as glyoxal oxidases (GLOX) and aryl alcohol oxidases (AAO), respectively. These enzymes are likely involved in lignin modification and potentially supply peroxidases and peroxygenases with required H2O2. Among the CAZy-classified proteins, one manganese peroxidase (MnP) (AA2; ID 221245) was found, which likely is involved in ligninolysis. Two heme-containing unspecific peroxygenases (UPOs; ID 226793, 183842), were also secreted, and as previously suggested they may play a role in ligninolytic processes, either by demethylation, hydroxylation, or potentially by contributing to the cleavage of β-O-4′ ether linkages.
HostingRepositoryPRIDE
AnnounceDate2024-03-15
AnnouncementXMLSubmission_2024-03-15_08:39:41.107.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD045077
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMirjam Kabel
SpeciesList scientific name: Agaricus bisporus var. bisporus H97; NCBI TaxID: 936046;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-04 04:31:45ID requested
12024-03-15 08:39:41announced
Publication List
10.6019/PXD045077;
Keyword List
submitter keyword: Enzymes, oxidoreductases, fungus, secretome, CAZy
Contact List
Mirjam A. Kabel
contact affiliationFood Chemistry, Wageningen Universiy & Research, The Netherlands
contact emailMirjam.kabel@wur.nl
lab head
Mirjam Kabel
contact affiliationAssociate professor, Wageningen University & Research; Food Chemistry
contact emailmirjam.kabel@wur.nl
dataset submitter
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Dataset FTP location
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