PXD045075

PXD045075 is an original dataset announced via ProteomeXchange.

Title	The role of inverse autotransporter FdeC in Avian Pathogenic Escherichia coli adhesion
Description	Avian Pathogenic Escherichia coli (APEC) poses a significant threat to the global poultry industry and has also zoonotic potential, meaning it could infect humans. One critical factor in APEC infection is its ability to adhere to host tissues, a process mediated by a protein called FdeC among others. This protein is present in pathogenic E. coli strains but is often nonfunctional in nonpathogenic ones. Our study focused on understanding the role of FdeC in APEC's ability to bind to chicken intestinal cells. Through an extensive screen of 2000 transposon mutants from the APEC IMT5155 strain, we discovered that a specific mutation in the fdeC gene led to enhanced adhesion. However, a direct deletion of the fdeC gene did not produce the same effect under standard conditions, which led us to investigate the environmental factors that might regulate FdeC expression. We found that FdeC's expression is highly dependent on the environmental conditions such as temperature and pH. Using optimized conditions for FdeC expression, we observed significantly higher adhesion and motility levels in the fdeC-deleted IMT5155 strain compared to the wild type. To understand the mechanisms behind this, we employed mass spectrometry and discovered that the absence of FdeC led to changes in ion transport and downregulated a protein called YbjN, which usually inhibits bacterial movement. Collectively, our findings suggest that FdeC has host-dependent expression and contributes to enhancement of bacterial fitness by modulating motility during colonization.
HostingRepository	PRIDE
AnnounceDate	2024-06-16
AnnouncementXML	Submission_2024-06-15_23:15:49.113.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fernando Garcia
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-04 03:59:26	ID requested
⏵ 1	2024-06-15 23:15:50	announced

10.1186/s13567-024-01327-5;

Aleksandrowicz A, Kj, æ, rup RB, Grzymaj, ł, o K, Martinez FG, Mu, ñ, oz J, Borowska D, Sives S, Vervelde L, Dalgaard TS, Kingsley RA, Kolenda R, FdeC expression regulates motility and adhesion of the avian pathogenic Escherichia coli strain IMT5155. Vet Res, 55(1):70(2024) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: Avian Patogenic Escheirichia. FdeC

Fernando Garcia Martinez
contact affiliation	Fernando Garcia Martinez Ph.D. Proteomics & MS Specialist Proteomics Unit, Spanish National Cancer Research Centre (CNIO) Melchor Fernández Almagro 3. E28029 MADRID, SPAIN. Phone +34 912246900 ext 3112
contact email	fgarcia@cnio.es
lab head
Fernando Garcia
contact affiliation	Fernando García Martínez, Ph.D. Proteomics Unit, Spanish National cancer Research Center, CNIO, MADRID, SPAIN
contact email	fgarcia@cnio.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD045075

PRIDE project URI

• PRIDE
  1. PXD045075
     1. Label: PRIDE project
     2. Name: The role of inverse autotransporter FdeC in Avian Pathogenic Escherichia coli adhesion