PXD045074

PXD045074 is an original dataset announced via ProteomeXchange.

Title	The tumour-derived extracellular vesicle proteome varies by endometrial cancer histology and is confounded by an obesogenic environment
Description	Endometrial cancer is the most prevalent gynaecological cancer globally. Its association with obesity and metabolic diseases is a key aetiology, increasingly among younger females. Early diagnosis and improved treatment decisions are crucial for these individuals which could be fulfilled by the discovery of new biomarkers. We took a new approach to extracellular vesicle (EV) biomarker discovery - profiling the proteome of enriched EVs isolated directly from frozen biobanked endometrial cancers. Nine tissue pools, each generating collagenase-digested tissue and matched small EVs, were analysed using label-free proteomics. Three clinical subgroups: Endometrioid low BMI (body mass index), Endometrioid high BMI, and Serous, irrespective of BMI were compared to identify shared secreted proteins, proteins associated with histological subtype, and proteins related to BMI. EVs were enriched for common EV markers and large secreted proteins. Cell lysates were enriched in mitochondrial and blood proteins. EV protein profiles were most different between the high BMI subgroup and the others, highlighting a significant influence of co-morbidities on the intra-tumoural EV secretome. Proteins that were differentially abundant between subgroups in tissues, were strikingly not also differential in the matched EVs. This work has identified secreted proteins implicated in the complex pathophysiology of endometrial cancer and pinpointed candidate biomarkers for diagnosis.
HostingRepository	PRIDE
AnnounceDate	2024-09-24
AnnouncementXML	Submission_2024-09-23_18:13:03.420.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045074
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	George, G GUO
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-04 03:28:08	ID requested
⏵ 1	2024-09-23 18:13:04	announced

10.1002/pmic.202300055;

Artuyants A, Guo G, Flinterman M, Middleditch M, Jacob B, Lee K, Vella L, Su H, Wilson M, Eva L, Shelling AN, Blenkiron C, The tumour-derived extracellular vesicle proteome varies by endometrial cancer histology and is confounded by an obesogenic environment. Proteomics, 24(11):e2300055(2024) [pubmed]

10.6019/PXD045074;

submitter keyword: SWATH,biomarkers, extracellular vesicles, endometrial cancer, secretome

Cherie Blenkiron
contact affiliation	Molecular Medicine and Pathology, The University of Auckland, NZ
contact email	c.blenkiron@auckland.ac.nz
lab head
George, G GUO
contact affiliation	The University of Auckland
contact email	guoguodigital@msn.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045074
     1. Label: PRIDE project
     2. Name: The tumour-derived extracellular vesicle proteome varies by endometrial cancer histology and is confounded by an obesogenic environment