PXD045071

PXD045071 is an original dataset announced via ProteomeXchange.

Title	Genetic and proteomic screens reveal perturbed nucleotide metabolism and DNA-protein crosslink formation as central mediators of decitabine cytotoxicity
Description	Decitabine (5-aza-dC) is a DNMT1-DPC inducing agent used to treat several hematological cancers. However, response rates vary, relapse is common, and predictive biomarkers are unknown. Here, we develop an adaptation of identification of proteins on nascent DNA (iPOND) by combining EdU with 5-aza-dC incorporation by itself or the presence of either ubiquitin E1 or SUMO E1 inhibitor which we term iPOND-DPC. The purpose of this experiments is to identify proteins that are recruited to DNMT1-DPCs and categorize whether their recruitment is dependent on SUMOylation or ubiquitylation.
HostingRepository	PRIDE
AnnounceDate	2024-04-18
AnnouncementXML	Submission_2024-04-18_07:07:07.181.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Petra Beli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-04 01:43:41	ID requested
⏵ 1	2024-04-18 07:07:07	announced

10.1038/S44318-024-00108-2;

submitter keyword: Decitabine,DNA-protein crosslink, TOPORS, global-genome DPC repair, DNA methylation, SUMO, DNMT1

Petra Beli
contact affiliation	Institute of Molecular Biology Mainz, Germany
contact email	p.beli@imb-mainz.de
lab head
Petra Beli
contact affiliation	Institute of Molecular Biology (IMB), Mainz, Germany
contact email	p.beli@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD045071

PRIDE project URI

• PRIDE
  1. PXD045071
     1. Label: PRIDE project
     2. Name: Genetic and proteomic screens reveal perturbed nucleotide metabolism and DNA-protein crosslink formation as central mediators of decitabine cytotoxicity