PXD045057

PXD045057 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of extracellular matrix (ECM) produced by human fibroblast activation protein (FAP)-positive pericyte-like cells and glioma cells
Description	Fibroblast activation protein (FAP) is upregulated in most cancers and represents an emerging theranostic target in oncology. In glioblastoma (GBM), FAP is predominantly expressed in mesenchymal pericyte-like cells, which contribute to GBM progression by promoting angiogenesis and glioma cell proliferation by soluble mediators. In this work, we show another possible mechanism by which these cells contribute to gliomagenesis. We observed that FAP+ pericyte-like cells residing in the perivascular niche in GBM are associated with elevated levels of the fibrillar extracellular matrix (ECM) proteins collagen I and fibronectin. Using sequential window acquisition of all theoretical mass spectra (SWATH-MS) analysis, we confirmed that the highest levels of collagen I and fibronectin are produced by FAP+ pericyte-like cells. In addition, we observed different expression pattern of basal membrane proteins between FAP+ pericyte-like cells and glioma cells.
HostingRepository	PanoramaPublic
AnnounceDate	2024-05-07
AnnouncementXML	Submission_2024-05-07_11:06:43.169.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jakub Cervenka
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Acetyl; Carbamidomethyl; Oxidation
Instrument	TripleTOF 5600+

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-03 15:20:54	ID requested
1	2024-05-02 12:48:42	announced
⏵ 2	2024-05-07 11:06:44	announced	: Added PubMed Id

Vymola P, Garcia-Borja E, Cervenka J, Balaziova E, Vymolova B, Veprkova J, Vodicka P, Skalnikova H, Tomas R, Netuka D, Busek P, Sedo A, Fibrillar extracellular matrix produced by pericyte-like cells facilitates glioma cell dissemination. Brain Pathol, 34(6):e13265(2024) [pubmed]

submitter keyword: Glioblastoma, Collagen I, Fibronectin, Fibroblast activation protein, Extracellular matrix, SWATH-MS

Petr Vodička
contact affiliation	Laboratory of Applied Proteome Analyses, Research Center PIGMOD, Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Libechov, Czech Republic
contact email	vodicka@iapg.cas.cz
lab head
Jakub Cervenka
contact affiliation	Laboratory of Applied Proteome Analyses, Research Center PIGMOD, Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Libechov, Czech Republic
contact email	cervenka@iapg.cas.cz
dataset submitter

Panorama Public dataset URI