PXD045050

PXD045050 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of different developmental stages of Cystoisospora suis (Apicomplexa: Coccidia) in vitro
Description	The apicomplexan parasite Cystoisospora suis has global significance as an enteropathogen of suckling piglets. Its intricate life cycle entails a transition from an asexual phase to sexual development, ultimately leading to the formation of transmissible oocysts. To advance our understanding of the parasite’s cellular development, we complemented previous transcriptome studies by delving into the proteome profiles at five distinct time points of in vitro cultivation through LC/MS-MS analysis. A total of 1324 proteins were identified in the in vitro developmental stages of C. suis, and 1082 proteins were identified as significantly differentially expressed. We performed BLAST, GO enrichment, and KEGG pathway analyses on the up- and downregulated proteins to elucidate correlated events in the C. suis life cycle. Our analyses revealed intriguing metabolic patterns in macromolecule metabolism, DNA and RNA-related processes, proteins associated with sexual stages, and those involved in cell invasion, reflecting the adaptation of sexual stages to a nutrient-poor and potentially stressful extracellular environment, with a focus on enzymes involved in metabolism and energy production. These findings have important implications for understanding the developmental biology of C. suis as well as other, related coccidian parasites, such as Eimeria spp. and Toxoplasma gondii. They also support the role of C. suis as a new model for the comparative biology of coccidian tissue cyst stages.
HostingRepository	PRIDE
AnnounceDate	2023-10-13
AnnouncementXML	Submission_2023-10-13_06:22:07.403.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TeresaCruz Bustos
SpeciesList	scientific name: Cystoisospora suis; NCBI TaxID: 483139;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-02 13:39:03	ID requested
⏵ 1	2023-10-13 06:22:07	announced

Dataset with its publication pending

submitter keyword: swine

Apicomplexa

sexual development

proteome

metabolism

oocyst

gametes,Isospora suis

Coccidia

anjajoachim
contact affiliation	institute parasitology, University of veterinary medicine vienna, Veterinärplatz 1, 1210 vienna, austria
contact email	Anja.Joachim@vetmeduni.ac.at
lab head
TeresaCruz Bustos
contact affiliation	Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210 Vienna,
contact email	teresa.cruz-bustos@vetmeduni.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD045050

PRIDE project URI

• PRIDE
  1. PXD045050
     1. Label: PRIDE project
     2. Name: Proteomic analysis of different developmental stages of Cystoisospora suis (Apicomplexa: Coccidia) in vitro