PXD045048 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The p53 Tumor Suppressor Regulates AKR1B1 Expression, a MetastasisPromoting Gene in Breast Cancer |
Description | Alteration of metabolism in cancer cells is a central aspect of the mechanisms that sustain aggressive traits. Aldo-keto reductase 1 B1 (AKR1B1) catalyzes the reduction of several aldehydes to alcohols consuming NADPH. Nevertheless, the ability of AKR1B1 to reduce different substrates renders difficult to comprehensively ascertain its biological role. Recent evidence has implicated AKR1B1 in cancer, however, the mechanisms underlying its pro-oncogenic function remain largely unknown. In this work, we report that AKR1B1 expression is controlled by the p53 tumor suppressor. We found that breast cancer patients bearing wild type TP53 have reduced AKR1B1 expression. In cancer cell lines, p53 reduced AKR1B1 mRNA and protein levels, and repressed promoter activity in luciferase assays. Furthermore, chromatin immunoprecipitation assays indicated that p53 is recruited to the AKR1B1 promoter. We also observed that AKR1B1 overexpression promoted metastasis in the 4T1 orthotopic model of triple negative breast cancer. Proteomic analysis of 4T1 cells overexpressing AKR1B1 showed that AKR1B1 exerts a marked effect on proteins related to metabolism, with particular impact on mitochondrial function. This work provides novel insights on the link between the p53 pathway and metabolism in cancer cells and contributes to characterizing the alterations associated to the pathologic role of AKR1B1. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:03:23.158.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Germán Rosano |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-02 09:02:55 | ID requested | |
1 | 2023-09-18 19:21:04 | announced | |
2 | 2023-11-14 09:01:21 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 06:03:23 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: breast cancer, Mitochondria, electron transport chain, aldo-keto reductases,AKR1B1, Proteomics, p53, metastasis |
Contact List
Germán Rosano |
contact affiliation | Instituto de Biología Molecular y Celular de Rosario, IBR,CONICET-UNR |
contact email | rosano@ibr-conicet.gov.ar |
lab head | |
Germán Rosano |
contact affiliation | Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET-UNR) |
contact email | rosano@ibr-conicet.gov.ar |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045048
- Label: PRIDE project
- Name: The p53 Tumor Suppressor Regulates AKR1B1 Expression, a MetastasisPromoting Gene in Breast Cancer