PXD045038

PXD045038 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic profiling of Arabidopsis nuclei reveals accumulation of nucleolar proteins after a prolonged heat stress
Description	Heat stress (HS) impacts the nuclear proteome and, subsequently, protein activities in the nucleoplasm and/or different nuclear compartments. In Arabidopsis thaliana, a short exposure to 37°C leads to the loss of the standard tripartite architecture of the nucleolus, the most prominent nuclear substructure, and, consequently, affects the assembly of ribosomes 1. Here, we report a quantitative label-free LC-MS/MS analysis to determine the nuclear proteome of Arabidopsis at 22ºC, HS (37ºC 4 and 24 hours), and a recovery phase. This analysis identified ten distinct groups of proteins based on their protein abundance in the nucleus before, during and after HS: the Early Group (EG), Late Group (LG), Transient Group (TG), Early Persistent Group (EPG), Late Persistent Group (LPG), Burst Group (BG), Early-Like Group (ELG), Late-Like Group (LLG), Transient-Like Group (TLG) and Continuous-Induced Group (CIG). Interestingly, the RNA Pol I subunit NRPA3 and other main nucleolar proteins – such as Nucleolin and Fibrillarin – were detected in the BG and CIG, suggesting that plants require increased nucleolar activity and likely ribosome assembly to restore protein synthesis after HS.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_04:00:29.446.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045038
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Magali Rompais
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	methionine oxidation with neutral loss of 80 Da; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-01 08:21:45	ID requested
⏵ 1	2024-10-17 04:00:30	announced

Mu, ñ, oz-D, í, az E, Fuenzalida-Valdivia I, Darri, è, re T, de Bures A, Blanco-Herrera F, Rompais M, Carapito C, S, á, ez-V, á, squez J, Proteomic profiling of Arabidopsis nuclei reveals distinct protein accumulation kinetics upon heat stress. Sci Rep, 14(1):18914(2024) [pubmed]

10.6019/PXD045038;

10.1038/s41598-024-65558-4;

submitter keyword: nanoLC-MSMS, Heat stress, nucleolus, nucleus

Christine CARAPITO
contact affiliation	Laboratoire de Spectrométrie de Masse Bio-Organique (LSMBO) Institut Pluridisciplinaire Hubert Curien (IPHC-UMR7178) CNRS - Université de Strasbourg Strasbourg, FRANCE
contact email	ccarapito@unistra.fr
lab head
Magali Rompais
contact affiliation	IPHC-UMR7178-CNRS-UDS-LSMBO
contact email	magali.rompais@unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045038
     1. Label: PRIDE project
     2. Name: Quantitative proteomic profiling of Arabidopsis nuclei reveals accumulation of nucleolar proteins after a prolonged heat stress