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PXD045036

PXD045036 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of Tau95 led to the identification of a four-subunit TFIIIC complex in trypanosomatid parasites
DescriptionRNA polymerase III (RNAP III) synthetizes small essential non-coding RNA molecules such as tRNAs and 5S rRNA. In yeast and vertebrates, RNAP III needs general transcription factors TFIIIA, TFIIIB and TFIIIC to initiate transcription. TFIIIC, composed of six subunits, binds to internal promoter elements in RNAP III-dependent genes. Limited information is available about RNAP III transcription in the trypanosomatid protozoa Trypanosoma brucei and Leishmania major, which diverged early from the eukaryotic lineage. Analyses of the first draft of the trypanosomatid genome sequences failed to recognize orthologs of any of the TFIIIC subunits, suggesting that this transcription factor is absent in these parasites. However, a single putative TFIIIC subunit was recently annotated in the databases. Here we characterize this subunit in T. brucei and L. major and demonstrate that it corresponds to Tau95. In silico analyses showed that both proteins possess the typical Tau95 sequences: the DNA binding region and the dimerization domain. As anticipated for a transcription factor, Tau95 localized to the nucleus in insect forms of both parasites. Chromatin immunoprecipitation (ChIP) assays demonstrated that Tau95 binds to tRNA and U2 snRNA genes in T. brucei. Remarkably, by performing tandem affinity purifications we identified orthologs of TFIIIC subunits Tau55, Tau131 and Tau138 in T. brucei and L. major. Thus, contrary to what was assumed, trypanosomatid parasites do possess a TFIIIC complex. Other putative interacting partners of Tau95 were identified in T. brucei and L. major.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:22:23.068.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD045036
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterEbbing de Jong
SpeciesList scientific name: Leishmania major strain Friedlin; NCBI TaxID: 347515; scientific name: Trypanosoma brucei brucei; NCBI TaxID: 5702;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-01 07:56:28ID requested
12024-01-16 07:36:02announced
22024-10-22 06:22:23announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD045036;
10.1007/S00253-023-12903-8;
Keyword List
submitter keyword: Leishmania major
Trypanosoma brucei
RNAP III transcription
TFIIIC
Tau95, Orbitrap Lumos
Contact List
Santiago Martinez-Calvillo
contact affiliationUnidad de Biomedicina Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México
contact emailscalv@unam.mx
lab head
Ebbing de Jong
contact affiliationSUNY Upstate Medical University
contact emaildejonge@upstate.edu
dataset submitter
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Dataset FTP location
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