PXD045036

PXD045036 is an original dataset announced via ProteomeXchange.

Title	Characterization of Tau95 led to the identification of a four-subunit TFIIIC complex in trypanosomatid parasites
Description	RNA polymerase III (RNAP III) synthetizes small essential non-coding RNA molecules such as tRNAs and 5S rRNA. In yeast and vertebrates, RNAP III needs general transcription factors TFIIIA, TFIIIB and TFIIIC to initiate transcription. TFIIIC, composed of six subunits, binds to internal promoter elements in RNAP III-dependent genes. Limited information is available about RNAP III transcription in the trypanosomatid protozoa Trypanosoma brucei and Leishmania major, which diverged early from the eukaryotic lineage. Analyses of the first draft of the trypanosomatid genome sequences failed to recognize orthologs of any of the TFIIIC subunits, suggesting that this transcription factor is absent in these parasites. However, a single putative TFIIIC subunit was recently annotated in the databases. Here we characterize this subunit in T. brucei and L. major and demonstrate that it corresponds to Tau95. In silico analyses showed that both proteins possess the typical Tau95 sequences: the DNA binding region and the dimerization domain. As anticipated for a transcription factor, Tau95 localized to the nucleus in insect forms of both parasites. Chromatin immunoprecipitation (ChIP) assays demonstrated that Tau95 binds to tRNA and U2 snRNA genes in T. brucei. Remarkably, by performing tandem affinity purifications we identified orthologs of TFIIIC subunits Tau55, Tau131 and Tau138 in T. brucei and L. major. Thus, contrary to what was assumed, trypanosomatid parasites do possess a TFIIIC complex. Other putative interacting partners of Tau95 were identified in T. brucei and L. major.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:22:23.068.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045036
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ebbing de Jong
SpeciesList	scientific name: Leishmania major strain Friedlin; NCBI TaxID: 347515; scientific name: Trypanosoma brucei brucei; NCBI TaxID: 5702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-01 07:56:28	ID requested
1	2024-01-16 07:36:02	announced
⏵ 2	2024-10-22 06:22:23	announced	2024-10-22: Updated project metadata.

10.6019/PXD045036;

10.1007/S00253-023-12903-8;

submitter keyword: Leishmania major

Trypanosoma brucei

RNAP III transcription

TFIIIC

Tau95, Orbitrap Lumos

Santiago Martinez-Calvillo
contact affiliation	Unidad de Biomedicina Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México
contact email	scalv@unam.mx
lab head
Ebbing de Jong
contact affiliation	SUNY Upstate Medical University
contact email	dejonge@upstate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045036
     1. Label: PRIDE project
     2. Name: Characterization of Tau95 led to the identification of a four-subunit TFIIIC complex in trypanosomatid parasites