PXD045013

PXD045013 is an original dataset announced via ProteomeXchange.

Title	Cysteine-rich with EGF-like domains 2 (CRELD2) is an endoplasmic reticulum stress-inducible angiogenic growth factor promoting ischemic heart repair
Description	Tissue repair after myocardial infarction (MI) is guided by incompletely defined autocrine and paracrine-acting proteins. Deciphering these signals and their upstream triggers is essential when considering infarct healing as a therapeutic target. Searching for previously unknown growth factors involved in infarct repair, we performed a bioinformatic secretome analysis in cardiac endothelial cells and identified cysteine-rich with EGF-like domains 2 (CRELD2), an endoplasmic reticulum stress-inducible protein with poorly characterized function. CRELD2 was abundantly expressed and secreted in the heart after MI in mice and patients. Creld2-deficient mice and wild-type mice treated with a CRELD2-neutralizing antibody displayed impaired de novo microvessel formation in the infarct border zone and developed severe postinfarction heart failure. CRELD2 protein therapy, conversely, improved heart function after MI. Exposing human coronary artery endothelial cells to recombinant CRELD2 induced angiogenesis, associated with a distinct phosphoproteome signature. These findings identify CRELD2 as a novel angiogenic growth factor and unravel a link between endoplasmic reticulum stress and ischemic tissue repair.
HostingRepository	PRIDE
AnnounceDate	2023-11-30
AnnouncementXML	Submission_2023-11-30_10:14:38.928.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felix Polten
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-31 17:56:44	ID requested
⏵ 1	2023-11-30 10:14:39	announced

Dataset with its publication pending

submitter keyword: Phosphoproteomics, Endothelial cells, Cysteine-rich with EGF-like domains 2, CRELD2, Myocardial Infarction,Tissue repair

Kai Christoph Wollert
contact affiliation	Hannover Medical School, Department of Cardiology and Angiology, Division of Molecular and Translational Cardiology, Hans Borst Center for Heart and Stem Cell Research, Hannover
contact email	Wollert.Kai@mh-hannover.de
lab head
Felix Polten
contact affiliation	Hanover Medical School - Division of Molecular and Translational Cardiology
contact email	Polten.Felix@mh-hannover.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045013
     1. Label: PRIDE project
     2. Name: Cysteine-rich with EGF-like domains 2 (CRELD2) is an endoplasmic reticulum stress-inducible angiogenic growth factor promoting ischemic heart repair