PXD045003

PXD045003 is an original dataset announced via ProteomeXchange.

Title	Global proteome of PTH1R transfected U2OS cells
Description	Brachydactyly type E (BDE), shortened metacarpals, metatarsals, cone-shaped epiphyses, and short stature, is an autosomal-dominant condition that commonly occurs as a sole phenotype. Parathyroid hormone-like protein (PTHrP) has been shown to be responsible in all forms to date, either directly or indirectly. We used linkage and then whole genome sequencing in a small pedigree, to elucidate BDE and identified a truncateddisintegrin-and-metalloproteinase-19 (ADAM19) allele in all affected family members, but not in nonaffected persons. Since we had shown earlier that the extracellular domain of the parathyroid hormone receptor (PTHR1) is subject to an unidentified metalloproteinase cleavage, we tested the hypothesis that ADAM19 is a sheddase for PTHR1. We transfected U2OS cells with ADAM19 (Control), PTH1R (disease-model), both PTH1R plus ADAM19 (healthy), and no transfection at all as another control. We did global proteome on these cell lines and compared them using a student t test.
HostingRepository	PRIDE
AnnounceDate	2024-02-15
AnnouncementXML	Submission_2024-02-15_07:05:37.717.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Trendelina Rrustemi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-31 07:11:36	ID requested
⏵ 1	2024-02-15 07:05:38	announced

Dataset with its publication pending

submitter keyword: ADAM19, Brachydactyly Type E,PTH1R, Global Proteome

Matthias Selbach
contact affiliation	Max Delbrueck Center for Molecular Medicine
contact email	matthias.selbach@mdc-berlin.de
lab head
Trendelina Rrustemi
contact affiliation	Max-Delbrück Center for Molecular Medicine
contact email	trendelina.rrustemi@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD045003

PRIDE project URI

• PRIDE
  1. PXD045003
     1. Label: PRIDE project
     2. Name: Global proteome of PTH1R transfected U2OS cells