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PXD044998

PXD044998 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMulti-omics, multi assay analysis of evolved resistance to ceftazidime-avibactam in Pseudomonas aeruginosa, compared to meropenem resistance and co-resistance evolution
DescriptionPseudomonas aeruginosa is a major cause of infection in hospitalised patients, with a large genome which makes it highly versatile and resistant to most antimicrobial agents. Ceftazidime-avibactam (CZA) offers an alternative treatment, but resistance is quickly evolving. There is limited knowledge on the exact resistance mechanisms to this drug, or on cross-resistance to meropenem (MEM). This laboratory experiment aimed to decipher these mechanisms in order to provide guidance for the best treatment choice in meropenem pre-treated P. aeruginosa infections. Six clinical isolates of P. aeruginosa were subjected to multistep resistance selection in sub inhibitory concentrations of CZA and MEM. MICs were also determined in the presence of the efflux pump inhibitor phenyl-Arginine-β-Naphthylamide (PAβN). Molecular analyses were performed by whole genome sequencing, whole -gene- and -protein expression profiles. CRISPR/Cas9 genome editing was performed using a two-plasmid method for selected mutations
HostingRepositoryPRIDE
AnnounceDate2025-05-21
AnnouncementXMLSubmission_2025-05-21_00:24:55.014.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAntje Dittmann
SpeciesList scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-31 05:09:23ID requested
12025-05-21 00:24:55announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, crispr/cas9, ceftazidime-avibactam, resistance mechanism,Pseudomonas aeruginosa, genomics, transcriptomics, dacB, meropenem
Contact List
Baharak Babouee Flury
contact affiliationMedical Research Center, Kantonspital St. Gallen, St. Gallen, Switzerland Division of Infectious Diseases and Hospital Epidemiology
contact emailbaharak.baboueeflury@kssg.ch
lab head
Antje Dittmann
contact affiliationUZH
contact emailantje.dittmann@gmail.com
dataset submitter
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Dataset FTP location
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