PXD044998 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Multi-omics, multi assay analysis of evolved resistance to ceftazidime-avibactam in Pseudomonas aeruginosa, compared to meropenem resistance and co-resistance evolution |
Description | Pseudomonas aeruginosa is a major cause of infection in hospitalised patients, with a large genome which makes it highly versatile and resistant to most antimicrobial agents. Ceftazidime-avibactam (CZA) offers an alternative treatment, but resistance is quickly evolving. There is limited knowledge on the exact resistance mechanisms to this drug, or on cross-resistance to meropenem (MEM). This laboratory experiment aimed to decipher these mechanisms in order to provide guidance for the best treatment choice in meropenem pre-treated P. aeruginosa infections. Six clinical isolates of P. aeruginosa were subjected to multistep resistance selection in sub inhibitory concentrations of CZA and MEM. MICs were also determined in the presence of the efflux pump inhibitor phenyl-Arginine-β-Naphthylamide (PAβN). Molecular analyses were performed by whole genome sequencing, whole -gene- and -protein expression profiles. CRISPR/Cas9 genome editing was performed using a two-plasmid method for selected mutations |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-21 |
AnnouncementXML | Submission_2025-05-21_00:24:55.014.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Antje Dittmann |
SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-31 05:09:23 | ID requested | |
⏵ 1 | 2025-05-21 00:24:55 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics, crispr/cas9, ceftazidime-avibactam, resistance mechanism,Pseudomonas aeruginosa, genomics, transcriptomics, dacB, meropenem |
Contact List
Baharak Babouee Flury |
contact affiliation | Medical Research Center, Kantonspital St. Gallen, St. Gallen, Switzerland Division of Infectious Diseases and Hospital Epidemiology |
contact email | baharak.baboueeflury@kssg.ch |
lab head | |
Antje Dittmann |
contact affiliation | UZH |
contact email | antje.dittmann@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044998
- Label: PRIDE project
- Name: Multi-omics, multi assay analysis of evolved resistance to ceftazidime-avibactam in Pseudomonas aeruginosa, compared to meropenem resistance and co-resistance evolution