PXD044998

PXD044998 is an original dataset announced via ProteomeXchange.

Title	Multi-omics, multi assay analysis of evolved resistance to ceftazidime-avibactam in Pseudomonas aeruginosa, compared to meropenem resistance and co-resistance evolution
Description	Pseudomonas aeruginosa is a major cause of infection in hospitalised patients, with a large genome which makes it highly versatile and resistant to most antimicrobial agents. Ceftazidime-avibactam (CZA) offers an alternative treatment, but resistance is quickly evolving. There is limited knowledge on the exact resistance mechanisms to this drug, or on cross-resistance to meropenem (MEM). This laboratory experiment aimed to decipher these mechanisms in order to provide guidance for the best treatment choice in meropenem pre-treated P. aeruginosa infections. Six clinical isolates of P. aeruginosa were subjected to multistep resistance selection in sub inhibitory concentrations of CZA and MEM. MICs were also determined in the presence of the efflux pump inhibitor phenyl-Arginine-β-Naphthylamide (PAβN). Molecular analyses were performed by whole genome sequencing, whole -gene- and -protein expression profiles. CRISPR/Cas9 genome editing was performed using a two-plasmid method for selected mutations
HostingRepository	PRIDE
AnnounceDate	2025-05-21
AnnouncementXML	Submission_2025-05-21_00:24:55.014.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antje Dittmann
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-31 05:09:23	ID requested
⏵ 1	2025-05-21 00:24:55	announced

Dataset with its publication pending

submitter keyword: proteomics, crispr/cas9, ceftazidime-avibactam, resistance mechanism,Pseudomonas aeruginosa, genomics, transcriptomics, dacB, meropenem

Baharak Babouee Flury
contact affiliation	Medical Research Center, Kantonspital St. Gallen, St. Gallen, Switzerland Division of Infectious Diseases and Hospital Epidemiology
contact email	baharak.baboueeflury@kssg.ch
lab head
Antje Dittmann
contact affiliation	UZH
contact email	antje.dittmann@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044998
     1. Label: PRIDE project
     2. Name: Multi-omics, multi assay analysis of evolved resistance to ceftazidime-avibactam in Pseudomonas aeruginosa, compared to meropenem resistance and co-resistance evolution