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PXD044994

PXD044994 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHepatitis C virus alters the morphology and function of peroxisomes.
DescriptionDespite the introduction of effective treatments for hepatitis C in clinics, issues remain regarding the liver diseases induced by chronic hepatitis C virus (HCV) infection. HCV is known to disturb the metabolism of infected cells, especially lipid metabolism and redox balance, but the mechanisms leading to HCV-induced pathogenesis are still poorly understood. In an APEX2-based proximity biotinylation screen, we identified ACBD5, a peroxisome membrane protein, as located in the vicinity of HCV replication complexes. Confocal microscopy confirmed the relocation of peroxisomes near HCV replication complexes and indicated that their morphology and number are altered in approximately 30% of infected Huh-7 cells. Peroxisomes are small versatile organelles involved among other functions in lipid metabolism and ROS regulation. To determine their importance in the HCV life cycle, we generated Huh-7 cells devoid of peroxisomes by inactivating the PEX5 and PEX3 genes using CRISPR/Cas9 and found that the absence of peroxisomes had no impact on replication kinetics or infectious titers of HCV strains JFH1 and DBN3a. The impact of HCV on peroxisomal functions was assessed using sub-genomic replicons. An increase of ROS was measured in peroxisomes of replicon-containing cells, and this increase was more pronounced than in mitochondria or the cytosol, suggesting that the redox balance of peroxisomes is specifically impaired in cells replicating HCV. Our study provides evidence that peroxisome function and morphology are altered in HCV-infected cells.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:07:26.400.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD044994
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSALIOU Jean-Michel
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-31 03:09:56ID requested
12023-10-24 11:22:21announced
22023-11-14 09:07:27announced2023-11-14: Updated project metadata.
Publication List
Martin de Fourchambault E, Callens N, Saliou JM, Fourcot M, Delos O, Barois N, Thorel Q, Ramirez S, Bukh J, Cocquerel L, Bertrand-Michel J, Marot G, Sebti Y, Dubuisson J, Rouill, é Y, Hepatitis C virus alters the morphology and function of peroxisomes. Front Microbiol, 14():1254728(2023) [pubmed]
10.6019/PXD044994;
Keyword List
submitter keyword: CRISPR-Cas9, peroxisome, APEX2, JFH1, DBN3a, proximity biotinylation, ROS,Hepatitis C virus, HCV genotype
Contact List
Jean-Michel Saliou
contact affiliationUniv. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US41 – UAR 2014 – PLBS, F-59000 Lille, France
contact emailjean-michel.saliou@pasteur-lille.fr
lab head
SALIOU Jean-Michel
contact affiliationInstitut Pasteur de Lille
contact emailjean-michel.saliou@pasteur-lille.fr
dataset submitter
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Dataset FTP location
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Repository Record List
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