PXD044994

PXD044994 is an original dataset announced via ProteomeXchange.

Title	Hepatitis C virus alters the morphology and function of peroxisomes.
Description	Despite the introduction of effective treatments for hepatitis C in clinics, issues remain regarding the liver diseases induced by chronic hepatitis C virus (HCV) infection. HCV is known to disturb the metabolism of infected cells, especially lipid metabolism and redox balance, but the mechanisms leading to HCV-induced pathogenesis are still poorly understood. In an APEX2-based proximity biotinylation screen, we identified ACBD5, a peroxisome membrane protein, as located in the vicinity of HCV replication complexes. Confocal microscopy confirmed the relocation of peroxisomes near HCV replication complexes and indicated that their morphology and number are altered in approximately 30% of infected Huh-7 cells. Peroxisomes are small versatile organelles involved among other functions in lipid metabolism and ROS regulation. To determine their importance in the HCV life cycle, we generated Huh-7 cells devoid of peroxisomes by inactivating the PEX5 and PEX3 genes using CRISPR/Cas9 and found that the absence of peroxisomes had no impact on replication kinetics or infectious titers of HCV strains JFH1 and DBN3a. The impact of HCV on peroxisomal functions was assessed using sub-genomic replicons. An increase of ROS was measured in peroxisomes of replicon-containing cells, and this increase was more pronounced than in mitochondria or the cytosol, suggesting that the redox balance of peroxisomes is specifically impaired in cells replicating HCV. Our study provides evidence that peroxisome function and morphology are altered in HCV-infected cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:07:26.400.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044994
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	SALIOU Jean-Michel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-31 03:09:56	ID requested
1	2023-10-24 11:22:21	announced
⏵ 2	2023-11-14 09:07:27	announced	2023-11-14: Updated project metadata.

Martin de Fourchambault E, Callens N, Saliou JM, Fourcot M, Delos O, Barois N, Thorel Q, Ramirez S, Bukh J, Cocquerel L, Bertrand-Michel J, Marot G, Sebti Y, Dubuisson J, Rouill, é Y, Hepatitis C virus alters the morphology and function of peroxisomes. Front Microbiol, 14():1254728(2023) [pubmed]

10.6019/PXD044994;

submitter keyword: CRISPR-Cas9, peroxisome, APEX2, JFH1, DBN3a, proximity biotinylation, ROS,Hepatitis C virus, HCV genotype

Jean-Michel Saliou
contact affiliation	Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US41 – UAR 2014 – PLBS, F-59000 Lille, France
contact email	jean-michel.saliou@pasteur-lille.fr
lab head
SALIOU Jean-Michel
contact affiliation	Institut Pasteur de Lille
contact email	jean-michel.saliou@pasteur-lille.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044994
     1. Label: PRIDE project
     2. Name: Hepatitis C virus alters the morphology and function of peroxisomes.