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PXD044993

PXD044993 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS analysis of isolated murine brain endothelial cells (EC) and full brain tissue (FT) preparations from 3-months-old mice followed by label-free quantification (LFQ)
DescriptionAge-related decline in brain endothelial cell (BEC) function critically contributes to cerebrovascular and neurodegenerative disease. Comprehensive atlases of the BEC transcriptome have become available but results from proteomic profiling are lacking. To gain insights into endothelial pathways affected by aging, we developed a magnetic-activated cell sorting (MACS)-based mouse BEC enrichment protocol compatible with high-resolution mass-spectrometry based proteomics. In this experiment, we have compared MACS sorted BEC and full brain tissue a massive enrichment of endothelial markers including Nos3, Cdh5, and Pecam1 in BECs compared to FT.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:29:52.745.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStephan Mueller
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-31 02:46:34ID requested
12024-02-15 01:30:13announced
22024-10-22 06:29:53announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: MACS,brain endothelial cells
Contact List
Stefan F. Lichtenthaler
contact affiliationDZNE Munich, Neuroproteomics, Feodor-Lynen Str. 17, 81377 Munich, Germany
contact emailstefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliationDZNE Munich Neuroproteomics
contact emailstephan.mueller@dzne.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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