PXD044985

PXD044985 is an original dataset announced via ProteomeXchange.

Title	Total porteome of liver tumors from mice (p53, MYC driven)
Description	The autophagy-lysosome system directs the degradation of a wide variety of cargo and is also involved in tumor progression. Here we show that immunity-related GTPase family Q protein (IRGQ) acts in the quality control of MHC-I molecules and directs misfolded MHC-I for lysosomal degradation through its binding to GABARAPL2 and LC3B. IRGQ specifically targets the non-conformational heavy chains of MHC-I, sorting them for degradation through the autophagy-lysosome system. In the absence of IRGQ, MHC-I heavy chains accumulate in the cell and partly get localized to the cell surface, thereby increasing interactions with mature MHC-I molecules and promoting immune response. Accordingly, mice suffering from hepatocellular carcinoma with reduced IRGQ levels display higher survival rates and bear more activated CD8+ T cells. Similarly, low IRGQ expression in human liver cancer correlates with higher levels of MHC-I molecules in the tumors, and patient survival is directly affected by IRGQ expression levels in CD8+ enriched tumors. Moreover, human T cells are more reactive toward tumorigenic IRGQ knock-out cells. Thus, we reveal IRGQ as a regulator of MHC-I quality control, mediating tumor immune evasion and marking it as a potential biomarker and therapeutic target.
HostingRepository	PRIDE
AnnounceDate	2024-10-31
AnnouncementXML	Submission_2024-10-31_06:06:36.460.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Uxia Gestal Mato
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-31 00:14:42	ID requested
⏵ 1	2024-10-31 06:06:36	announced

10.1016/J.CELL.2024.09.048;

submitter keyword: TMT,Liver, MYC, Mice, p53

Lina Herhaus
contact affiliation	Institute of Biochemistry II Goethe University Medical School University Hospital, Bldg 75 Theodor-Stern-Kai 7 60590 Frankfurt am Main Germany
contact email	herhaus@em.uni-frankfurt.de
lab head
Uxia Gestal Mato
contact affiliation	Institut für Biochemie II - Goethe Universität Frankfurt
contact email	gestalmato@em.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD044985

PRIDE project URI

• PRIDE
  1. PXD044985
     1. Label: PRIDE project
     2. Name: Total porteome of liver tumors from mice (p53, MYC driven)