PXD044977

PXD044977 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Chemoproteomic analysis of PFI-7 specificity for target GID4
Description	PFI-7 is a GID4 specific inhibitor. In order to verify the specificity of GID4 towards the target, a chemoproteomic competition experiment was performed. The biotinylated adduct of PFI-7, NB716, is used to enrich for potential target proteins of PFI-7. When PFI-7 is added in excess, the inhibitor competes with NB716 for target binding. We demonstrate that GID4 is the only protein that is affected by addition of PFI-7, and we observe no effects when adding the negative control to PFI-7, PFI-7N.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:39:37.456.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Viviane Reber
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-30 09:55:53	ID requested
1	2024-05-17 03:43:22	announced
⏵ 2	2024-10-22 06:39:37	announced	2024-10-22: Updated project metadata.

Publication List

10.1038/S41589-024-01618-0;

10.1038/S41589-024-01618-0;

Keyword List

submitter keyword: target engagement,Chemoproteomics, specificity, PFI-7

submitter keyword: target engagement,Chemoproteomics, specificity, PFI-7

Contact List

Matthias Gstaiger
contact affiliation	Institute of Molecular Systems Biology at ETH Zurich, Zurich, Switzerland
contact email	gstaigem@ethz.ch
lab head
Viviane Reber
contact affiliation	IMSB, ETH Zurich
contact email	reberv@ethz.ch
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD044977
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD044977

PRIDE project URI

Repository Record List

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