PXD044972

PXD044972 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis Total Leaf Proteomics Probing Amino Acid Misincorporation
Description	Protein translation is a fundamental principle in cell biology. It requires transfer RNAs (tRNAs) loaded with their cognate amino acid to ensure the accurate incorporation of amino acid into the nascent peptide chain. Bacteria, mitochondria, and plastids synthesize glutaminyl -tRNAs (Gln-tRNAGln) for protein synthesis via an indirect pathway. tRNAGln is initially charged with glutamate by a non-discriminating aminoacyl-tRNA synthetase. Subsequently, tRNA-attached glutamine is produced by transamidation of the Glu-tRNAGln via the aminoacyl-tRNA amido-transferase complex GatCAB. Impaired function of amido-transferases has mostly negative effects in humans, yeast and bacteria. Interestingly however, in bacteria protein mistranslation through changed amido-transferase activity was shown as specialized strategy to survive adverse conditions, such as reactive oxygen stress, UV-light or inhibitor treatments. The consequences of decreased amido-transferase activity in plant and especially its plastids are unknown. Tampering with the plant amido-transferase should result in amino acid misincorporation for proteins synthesized in plastids and mitochondria while nuclear encoded proteins should not exhibit increased rate of Gln to Glu substitutions. Using this dataset, we aim to investigate this hypothesis.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-08_02:19:05.368.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Brandt
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	acetylated residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-30 08:20:53	ID requested
⏵ 1	2026-06-08 02:19:05	announced

10.1073/PNAS.2537357123;

submitter keyword: Arabidopsis, Amino acid Minsincorporation, Total Leaf Protein

Prof. Hans-Henning
contact affiliation	LMU Munich Plant Biochemistry and Physiology, Germany
contact email	kunz@lmu.de
lab head
Benjamin Brandt
contact affiliation	LMU Munich Plant Biochemistry and Physiology
contact email	brandt@lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044972
     1. Label: PRIDE project
     2. Name: Arabidopsis Total Leaf Proteomics Probing Amino Acid Misincorporation