PXD044962

PXD044962 is an original dataset announced via ProteomeXchange.

Title	Differential contributions of CodY, SigB and the hibernation promoting factor HPF to interactions of Staphylococcus aureus USA300 with human lung epithelial cells explained by proteome analysis
Description	The Gram-positive bacterium Staphylococcus aureus is a leading cause of severe pneumonia. Therefore, we recently investigated the fate of S. aureus upon internalization by human lung epithelial cells. This uncovered three major adaptive responses of the bacteria, involving the SigB and CodY regulons and an upregulation of the S. aureus hibernation-promoting factor (SaHPF). To explore the roles of CodY, SigB and SaHPF in the infection of human lung epithelial cells, we employed derivatives of the community-acquired MRSA strain USA300 with transposon mutations in the respective genes. Interestingly, the investigated codY mutant bacteria displayed a ‘small colony variant’-like phenotype that promotes their intracellular survival and localizes the bacteria to the host cell cytoplasm. Furthermore, our results show that CodY, SigB and saHPF contribute differentially to the different steps in the infectious process, including, host cell adhesion, invasion, intracellular survival and cytotoxicity. Notably, codY or sigB mutant bacteria experienced faster intracellular clearance compared to the parental strain, underscoring the importance of these regulators for intracellular persistence. Furthermore, we show an unprecedented role of SaHPF in human lung epithelial cell infection, including parallel effects of saHPF or SigB deficiency in adhesion and invasion. Altogether, our study focuses attention on the CodY-perceived metabolic state of the bacteria and the SigB-perceived stress in bacterial decision taking during infection. Conversely, our observations indicate important roles for the nutritional status and bacterial stress-inducing conditions in the host for the onset and subsequent course of invasive or chronic staphylococcal lung infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:57:55.997.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Larissa Busch
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-30 06:18:25	ID requested
1	2024-09-10 23:25:44	announced
⏵ 2	2024-10-22 06:57:56	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: epithel cells, USA300, hibernation promoting factor,Staphylococcus aureus

Uwe Völker
contact affiliation	Interfaculty Institute of Genetics and Functional Genomics, Department of Functional Genomics, University Medicine Greifswald, Germany
contact email	voelker@uni-greifswald.de
lab head
Larissa Busch
contact affiliation	Department of Functional Genomics, University Medicine Greifswald
contact email	larissa.busch@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044962
     1. Label: PRIDE project
     2. Name: Differential contributions of CodY, SigB and the hibernation promoting factor HPF to interactions of Staphylococcus aureus USA300 with human lung epithelial cells explained by proteome analysis