PXD044945

PXD044945 is an original dataset announced via ProteomeXchange.

Title	Metal organic layers enabled cell surface engineering coupling biomembrane fusion for dynamic membrane proteome profiling
Description	Systematically dissecting the highly dynamic and tightly communicating membrane proteome of living cells is essential for systems-level understanding of fundamental cellular processes and intricate relationship between membrane-bound organelles constructed through membrane traffic. While extensive efforts have been made to enrich membrane proteins, the comprehensive analysis of them with high selectivity and deep coverage remains a challenge, especially at the living cell state. To address this problem, we developed the cell surface engineering coupling biomembrane fusion method to map the whole membrane proteome from plasma membrane to various organelle membranes taking advantage of the exquisite interaction between two-dimensional metal-organic layers and phospholipid bilayers on membrane. This approach, which bypassed conventional biochemical fractionation and ultracentrifugation, facilitated the enrichment of membrane proteins in their native phospholipid bilayer environment, helping map the membrane proteome with a specificity of 77% and realizing the deep coverage of the HeLa membrane proteome (5,087 membrane proteins). Furthermore, membrane N-phosphoproteome was profiled by integrating N-phosphoproteome analysis strategy and dynamic membrane proteome during apoptosis was deciphered in combination with quantitative proteomics, the features of membrane protein N-phosphorylation modifications and many differential proteins during apoptosis associated with mitochondrial dynamics, ER homeostasis were found. The method provided a simple and robust strategy for efficient analysis of membrane proteome, offered a reliable platform for research on membrane -related cell dynamic events and expanded the application of metal-organic layers.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:09:32.372.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qianqian Jiang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Velos; Orbitrap Exploris 480; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-29 19:32:36	ID requested
⏵ 1	2024-01-26 07:09:33	announced

10.1039/d3sc03725h;

Jiang Q, Wang H, Qiao Z, Hou Y, Sui Z, Zhao B, Liang Z, Jiang B, Zhang Y, Zhang L, Metal organic layers enabled cell surface engineering coupling biomembrane fusion for dynamic membrane proteome profiling. Chem Sci, 14(42):11727-11736(2023) [pubmed]

submitter keyword: Membrane protein；enrichment；metal organic layers；proteome

Qianqian Jiang
contact affiliation	CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
contact email	jiangqq@dicp.ac.cn
lab head
Qianqian Jiang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	jiangqq1810@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD044945

PRIDE project URI

• PRIDE
  1. PXD044945
     1. Label: PRIDE project
     2. Name: Metal organic layers enabled cell surface engineering coupling biomembrane fusion for dynamic membrane proteome profiling