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PXD044937

PXD044937 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMultiomics Sample Preparation Workflow for Simultaneous Cleanup of DNA, RNA, and Proteins using ProMTag
DescriptionTypical multiomics studies employ separate methods for DNA, RNA, and protein sample preparation, which is labor intensive, costly, and prone to sampling bias. We describe here a method for preparing high-quality DNA, RNA, and protein or peptides for whole genome sequencing, RNA sequencing, and whole proteome analysis from a single sample. This method utilizes a reversible protein tagging scheme to covalently link all proteins in a lysate to a bead-based matrix and nucleic acid precipitation and selective solubilization to yield separate pools of protein and nucleic acids. The proteins are then digested to generate peptides ready for mass spectrometric proteome analysis. We demonstrate the utility of this method to compare the genomes, transcriptomes, and proteomes of four triple-negative breast cancer cell lines with different degrees of malignancy. These data show the involvement of both RNA and associated proteins, and protein-only dependent pathways that distinguish these cell lines. We also demonstrate the utility of this multiomics workflow for tissue analysis using mouse brain, liver and lung tissue.
HostingRepositoryMassIVE
AnnounceDate2025-02-20
AnnouncementXMLSubmission_2025-02-20_11:24:51.682.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStephanie Byrum
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListOxidation
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-29 10:38:17ID requested
12025-02-20 11:24:52announced
Publication List
no publication
Keyword List
submitter keyword: multi-omics, triple-negative breast cancer
Contact List
Jonathan Minden
contact affiliationCarnegie Mellon University
contact emailminden@andrew.cmu.edu
lab head
Stephanie Byrum
contact affiliationSt Jude Children's Research Hospital
contact emailsbyrum@stjude.org
dataset submitter
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MassIVE dataset URI
Dataset FTP location
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