PXD044913 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparison of extracellular vesicle proteome between glaucoma and non-glaucoma trabecular meshwork cells |
Description | Extracellular matrix (ECM) materials accumulate in the trabecular meshwork (TM) tissue of patients with glaucoma, which is associated with a decrease in aqueous humor outflow and therefore an increase in intraocular pressure. To explore a potential mechanism for ECM regulation in the TM, we purified extracellular vesicles (EVs) from conditioned media of differentiated TM cells in culture isolated from non-glaucomatous and glaucomatous human donor eyes. EVs were purified using the double cushion ultracentrifugation gradient method. Fractions containing EV markers CD9 and TSG101 were analyzed using nanoparticle tracking analysis to determine their size and concentration. We then determined their proteomic cargo by mass spectrometry and compared protein profiles of EVs between normal and glaucomatous TM cells using PANTHER. Key protein components from EV preparations were validated with Western blotting. Results showed changes in the percentage of ECM proteins associated with EVs from glaucomatous TM cells compared to non-glaucomatous TM cells (5.7% vs 13.1% respectively). Correspondingly, we found that two ECM-related cargo proteins found across all samples, fibronection and EDIL3 were significantly less abundant in glaucomatous EVs (<0.3 fold change across all groups) compared to non-glaucomatous EVs. Overall, these data establish that ECM materials are prominent cargo in EVs from TM cells, and their binding to EVs is diminished in glaucoma |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-26 |
AnnouncementXML | Submission_2024-01-26_06:13:53.503.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD044913 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Nik Skiba |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-28 10:55:18 | ID requested | |
⏵ 1 | 2024-01-26 06:13:54 | announced | |
Publication List
McDonnell FS, Riddick BJ, Roberts H, Skiba N, Stamer WD, Comparison of the extracellular vesicle proteome between glaucoma and non-glaucoma trabecular meshwork cells. Front Ophthalmol (Lausanne), 3():(2023) [pubmed] |
10.6019/PXD044913; |
10.3389/fopht.2023.1257737; |
Keyword List
submitter keyword: proteomics, ete, extracellular vesicles, trabecular meshwork,human |
Contact List
W. Daniel Stamer |
contact affiliation | Duke University, Eyer Centetr |
contact email | william.stamer@duke.edu |
lab head | |
Nik Skiba |
contact affiliation | Duke University |
contact email | skibanik@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044913
- Label: PRIDE project
- Name: Comparison of extracellular vesicle proteome between glaucoma and non-glaucoma trabecular meshwork cells