PXD044903

PXD044903 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial proteome analysis reveals that an augmented cytochrome c oxidase assembly and activity potentiates respiratory capacity in sarcoma
Description	Sarcoma is a type of cancer that developed from transformed mesenchymal cells like bone, cartilage, fat, muscle, etc. and is broadly categorized into two types, bone and soft tissue sarcoma. Solid tumor cells rely on glycolysis for energy production, contrary to the general belief, it was previously reported that sarcoma cells rely on mitochondrial respiration and the respiratory chain inhibitors drugs such as methylglyoxal, metformin, etc. selectively kill sarcoma cells. Therefore, the study of detailed mitochondrial proteomes is essential to understand the basic metabolic requirement of sarcoma mitochondria. Here, we generate a solid fibrosarcoma mouse tumor model by injecting a chemical carcinogen 3-methyl cholanthrene into the left hind leg. Differential proteomic profiling of ultrapure mitochondria from mice fibrosarcoma tissues (ST, n=4) and its contralateral leg muscle tissues (CLT, n=4) was performed using an LTQ orbitrap XL mass spectrometer. We found ~429 mitochondria-associated protein hits in a total of eight mice tissue samples. Most of the mitochondrial respiratory chain (MRC) subunits were unaltered, whereas two mitochondrial complex IV i.e. cytochrome c oxidase subunits were upregulated.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:39:26.040.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044903
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	ALOK GHOSH
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-28 01:59:05	ID requested
⏵ 1	2024-10-17 02:39:26	announced

10.1016/j.bbrc.2024.150501;

10.6019/PXD044903;

Bedi M, Das S, Das J, Mukherjee S, Basu A, Saha S, Ghosh A, Mitochondrial proteome analysis reveals that an augmented cytochrome c oxidase assembly and activity potentiates respiratory capacity in sarcoma. Biochem Biophys Res Commun, 736():150501(2024) [pubmed]

submitter keyword: Sarcoma, and respiratory chain subunits, differential proteomics, mitochondria

Dr. Alok Ghosh
contact affiliation	Assistant Professor, Department of Biochemistry, University of Calcutta, Kolkata, India
contact email	alok.caluni@gmail.com
lab head
ALOK GHOSH
contact affiliation	ASSISTANT PROFESSOR
contact email	alok.caluni@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD044903

PRIDE project URI

• PRIDE
  1. PXD044903
     1. Label: PRIDE project
     2. Name: Mitochondrial proteome analysis reveals that an augmented cytochrome c oxidase assembly and activity potentiates respiratory capacity in sarcoma