<<< Full experiment listing

PXD044903

PXD044903 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMitochondrial proteome analysis reveals that an augmented cytochrome c oxidase assembly and activity potentiates respiratory capacity in sarcoma
DescriptionSarcoma is a type of cancer that developed from transformed mesenchymal cells like bone, cartilage, fat, muscle, etc. and is broadly categorized into two types, bone and soft tissue sarcoma. Solid tumor cells rely on glycolysis for energy production, contrary to the general belief, it was previously reported that sarcoma cells rely on mitochondrial respiration and the respiratory chain inhibitors drugs such as methylglyoxal, metformin, etc. selectively kill sarcoma cells. Therefore, the study of detailed mitochondrial proteomes is essential to understand the basic metabolic requirement of sarcoma mitochondria. Here, we generate a solid fibrosarcoma mouse tumor model by injecting a chemical carcinogen 3-methyl cholanthrene into the left hind leg. Differential proteomic profiling of ultrapure mitochondria from mice fibrosarcoma tissues (ST, n=4) and its contralateral leg muscle tissues (CLT, n=4) was performed using an LTQ orbitrap XL mass spectrometer. We found ~429 mitochondria-associated protein hits in a total of eight mice tissue samples. Most of the mitochondrial respiratory chain (MRC) subunits were unaltered, whereas two mitochondrial complex IV i.e. cytochrome c oxidase subunits were upregulated.
HostingRepositoryPRIDE
AnnounceDate2024-10-17
AnnouncementXMLSubmission_2024-10-17_02:39:26.040.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD044903
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterALOK GHOSH
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap XL
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-28 01:59:05ID requested
12024-10-17 02:39:26announced
Publication List
10.1016/j.bbrc.2024.150501;
10.6019/PXD044903;
Bedi M, Das S, Das J, Mukherjee S, Basu A, Saha S, Ghosh A, Mitochondrial proteome analysis reveals that an augmented cytochrome c oxidase assembly and activity potentiates respiratory capacity in sarcoma. Biochem Biophys Res Commun, 736():150501(2024) [pubmed]
Keyword List
submitter keyword: Sarcoma, and respiratory chain subunits, differential proteomics, mitochondria
Contact List
Dr. Alok Ghosh
contact affiliationAssistant Professor, Department of Biochemistry, University of Calcutta, Kolkata, India
contact emailalok.caluni@gmail.com
lab head
ALOK GHOSH
contact affiliationASSISTANT PROFESSOR
contact emailalok.caluni@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD044903
PRIDE project URI
Repository Record List
[ + ]