PXD044862

PXD044862 is an original dataset announced via ProteomeXchange.

Title	SART3 is a Novel Rme2s Reader studied by tandem MS
Description	Arginine methylation is a ubiquitous post-translational modification involved in many biological processes such as transcription, cell signaling and RNA splicing. Tudor domains by far are the major effector domain family for arginine methylation mark recognition. Here we characterize SART3 as a novel selective reader for symmetric dimethylarginine (Rme2s) marks. SART3 harbors a series of HAT (Half-a-TPR) repeats that are aromatic-rich, and it is this region that binds Rme2s motifs. An analysis of the reported structure of the HAT repeats of SART3 identified a putative aromatic cage that potentially “read” the Rme2s-modified motifs, and the key aromatic residues required for Rme2s recognition were identified. We further confirm that this Rme2s reader ability is important for SART3-mediated RNA splicing. Together, our studies revealed that the evolutionarily conserved SART3 HAT domain was a novel reader of Rme2s mark involved in RNA splicing.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:41:33.187.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Person
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-25 09:22:06	ID requested
⏵ 1	2024-10-17 02:41:34	announced

10.1016/j.celrep.2024.114459;

Wang Y, Zhou J, He W, Fu R, Shi L, Dang NK, Liu B, Xu H, Cheng X, Bedford MT, SART3 reads methylarginine-marked glycine- and arginine-rich motifs. Cell Rep, 43(7):114459(2024) [pubmed]

submitter keyword: SART3, HAT(half-a-TPR), RNA splicing, Rme2s reader, arginine methylation

Mark T. Bedford
contact affiliation	Professor, Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Houston, TX
contact email	mtbedford@mdanderson.org
lab head
Maria Person
contact affiliation	University of Texas at Austin
contact email	mperson@austin.utexas.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044862
     1. Label: PRIDE project
     2. Name: SART3 is a Novel Rme2s Reader studied by tandem MS