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PXD044862

PXD044862 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSART3 is a Novel Rme2s Reader studied by tandem MS
DescriptionArginine methylation is a ubiquitous post-translational modification involved in many biological processes such as transcription, cell signaling and RNA splicing. Tudor domains by far are the major effector domain family for arginine methylation mark recognition. Here we characterize SART3 as a novel selective reader for symmetric dimethylarginine (Rme2s) marks. SART3 harbors a series of HAT (Half-a-TPR) repeats that are aromatic-rich, and it is this region that binds Rme2s motifs. An analysis of the reported structure of the HAT repeats of SART3 identified a putative aromatic cage that potentially “read” the Rme2s-modified motifs, and the key aromatic residues required for Rme2s recognition were identified. We further confirm that this Rme2s reader ability is important for SART3-mediated RNA splicing. Together, our studies revealed that the evolutionarily conserved SART3 HAT domain was a novel reader of Rme2s mark involved in RNA splicing.
HostingRepositoryPRIDE
AnnounceDate2024-10-17
AnnouncementXMLSubmission_2024-10-17_02:41:33.187.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaria Person
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-25 09:22:06ID requested
12024-10-17 02:41:34announced
Publication List
10.1016/j.celrep.2024.114459;
Wang Y, Zhou J, He W, Fu R, Shi L, Dang NK, Liu B, Xu H, Cheng X, Bedford MT, SART3 reads methylarginine-marked glycine- and arginine-rich motifs. Cell Rep, 43(7):114459(2024) [pubmed]
Keyword List
submitter keyword: SART3, HAT(half-a-TPR), RNA splicing, Rme2s reader, arginine methylation
Contact List
Mark T. Bedford
contact affiliationProfessor, Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Houston, TX
contact emailmtbedford@mdanderson.org
lab head
Maria Person
contact affiliationUniversity of Texas at Austin
contact emailmperson@austin.utexas.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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