PXD044858

PXD044858 is an original dataset announced via ProteomeXchange.

Title	Oral exposure to bisphenol S is associated with alterations in oviduct proteome with aggravated effects in overweight females in an ovine model
Description	In mammals, the final maturation and transport of gametes, fertilization and early embryo development take place in the oviducts. Bisphenol S (BPS) is a substitute for bisphenol A in plastic manufacturing and, as a potential endocrine disruptor, may alter the oviduct physiology. Furthermore, bisphenols are fat-soluble and can accumulate in fatty tissues. The objective of this study was to assess the effect of a daily dietary exposure to BPS combined with a contrasted diet on the oviduct fluid (OF) proteome using an ovine model. Eighty adult cyclic ewes were allotted to four groups (20/group): over-fed consuming in their diet 50 µg/kg/day of BPS, under-fed consuming 50 µg/kg/day of BPS, and non-exposed controls in each diet group. After three months, the mean body condition score and plasma levels of glucose and non-esterified fatty acids were significantly higher in over- than in under-fed females. The proteins in collected OF samples (50 µg) were analyzed by nanoliquid chromatography coupled with tandem mass spectrometry (nanoLC-MS/MS). Overall, 1563 proteins were identified, among which 848 were quantified. Principal component analysis of data revealed a clear discrimination of samples according to the diet and a segregation between BPS-exposed and non-exposed females in over-fed ewes. Hierarchical clustering of differentially abundant proteins (DAPs) identified two clusters of 101 and 78 DAPs according to the diet. Pairwise comparisons between groups revealed a stronger effect of BPS in over- than in under-fed females (70 vs. 24 DAPs) and a stronger effect of the diet in BPS-exposed than non-exposed females (56 vs. 36 DAPs). Functional analysis of DAPs showed an enrichment in metabolic processes, immune system, cell response to stress, and reproductive processes. This work highlights for the first time the important impact of BPS on the oviduct proteome with bigger effects in over- than underweight females.
HostingRepository	PRIDE
AnnounceDate	2024-05-28
AnnouncementXML	Submission_2024-05-28_06:09:53.617.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valerie LABAS
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-25 07:01:42	ID requested
⏵ 1	2024-05-28 06:09:54	announced

Dataset with its publication pending

submitter keyword: ovine, oviduct, bisphenol s,fluid, GeLC-MS/MS

Marie Saint-Dizier
contact affiliation	INRAE Centre Val de Loire UMR Physiologie de la Reproduction et des Comportements UMR INRAE 85-CNRS 7247-Université de Tours -IFCE 37380 Nouzilly France
contact email	marie.saint-dizier@univ-tours.fr
lab head
Valerie LABAS
contact affiliation	INRAE
contact email	valerie.labas@inrae.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD044858

PRIDE project URI

• PRIDE
  1. PXD044858
     1. Label: PRIDE project
     2. Name: Oral exposure to bisphenol S is associated with alterations in oviduct proteome with aggravated effects in overweight females in an ovine model